PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Malic Acid Interacts with Citric Acid to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Chinese Water Chestnut Residue and Corn Gluten Meal
Version 1
: Received: 9 December 2023 / Approved: 11 December 2023 / Online: 11 December 2023 (09:32:44 CET)
How to cite:
Zhao, C.; Li, Y.; Chen, Q.; Guo, Y.; Sun, B.; Liu, D. Malic Acid Interacts with Citric Acid to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Chinese Water Chestnut Residue and Corn Gluten Meal. Preprints2023, 2023120685. https://doi.org/10.20944/preprints202312.0685.v1
Zhao, C.; Li, Y.; Chen, Q.; Guo, Y.; Sun, B.; Liu, D. Malic Acid Interacts with Citric Acid to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Chinese Water Chestnut Residue and Corn Gluten Meal. Preprints 2023, 2023120685. https://doi.org/10.20944/preprints202312.0685.v1
Zhao, C.; Li, Y.; Chen, Q.; Guo, Y.; Sun, B.; Liu, D. Malic Acid Interacts with Citric Acid to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Chinese Water Chestnut Residue and Corn Gluten Meal. Preprints2023, 2023120685. https://doi.org/10.20944/preprints202312.0685.v1
APA Style
Zhao, C., Li, Y., Chen, Q., Guo, Y., Sun, B., & Liu, D. (2023). Malic Acid Interacts with Citric Acid to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Chinese Water Chestnut Residue and Corn Gluten Meal. Preprints. https://doi.org/10.20944/preprints202312.0685.v1
Chicago/Turabian Style
Zhao, C., Baoli Sun and Dewu Liu. 2023 "Malic Acid Interacts with Citric Acid to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Chinese Water Chestnut Residue and Corn Gluten Meal" Preprints. https://doi.org/10.20944/preprints202312.0685.v1
Abstract
This experiment aimed to investigate the impact of malic acid (MA) and citric acid (CA) on the nutritional composition, fermentation quality, rumen degradation rate, and microbial diversity of a mixture of apple pomace and corn protein powder during ensiling. The experiment used apple pomace and corn protein powder as raw materials, with four groups: control group (CON), malic acid treatment group (MA, 10g/kg), citric acid treatment group (CA, 10g/kg), and citric acid + malic acid treatment group (MA, 10g/kg + CA, 10g/kg), subjected to mixed ensiling for 60 days. The results indicated: (1) Compared to the CON group, the crude protein content significantly increased in the MA, CA, and MA+CA groups (p < 0.05), with the highest content observed in the MA+CA group. The addition of MA and CA effectively reduced the water-soluble carbohydrate (WSC) content (p < 0.05). Simultaneously, the CA group showed a decreasing trend in NDFom and hemicellulose content (p = 0.08; p = 0.09). (2) Compared to the CON group, the pH significantly decreased in the MA, CA, and MA+CA groups (p < 0.01), and the three treatment groups exhibited a significant increase in lactic acid and acetic acid content (p < 0.01). The quantity of lactic acid bacteria increased significantly (p < 0.01), with the MA+CA group showing a more significant increase than the MA and CA groups (p < 0.05). (3) Compared to the CON group, the in situ dry matter disappearance (ISDMD) significantly increased in the MA, CA, and MA+CA groups (p < 0.05). All three treatment groups showed highly significant differences in in situ crude protein disappearance (ISCPD) compared to the CON group (p < 0.01). (4) Good's Coverage for all experimental groups was greater than 0.99, meeting the conditions for subsequent sequencing. Compared to the CON group, the Shannon index significantly increased in the CA group (P < 0.01), and the Simpson index increased significantly in the MA group (p < 0.05). However, there was no significant difference in the Chao index among the three treatment groups and the CON group (p > 0.05). At the genus level, the abundance of Lentilactobacillus in the MA, CA, and MA+CA groups was significantly higher than in the control group (p < 0.05). PICRUSt prediction results indicated that the metabolic functional microbial groups in the CA and MA treatment groups were significantly higher than in the CON group (p < 0.05), suggesting that the addition of MA or CA could reduce the loss of nutritional components such as protein and carbohydrates in mixed ensilage. In conclusion, the addition of malic acid and citric acid to a mixture of apple pomace and corn protein powder during ensiling reduces nutritional losses, improves fermentation quality and rumen degradation rate, enhances the diversity of the microbial community in ensiled feed, and improves microbial structure. The combined addition of malic acid and citric acid demonstrates a superior effect.
Keywords
chinese water chestnut; corn gluten meal; malic acid; citric acid; mixed silage; microbial community
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
