Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Title Novel Insights into the Neuroprotective Attributes of Polyamines in Selected Edible and Medicinal Fungi: A Pioneering Report

Milena Rašeta
* ORCID logo ,
Marko Kebert
ORCID logo ,
Jovana Mišković
ORCID logo ,
Milana Rakić
ORCID logo ,
Saša Kostić
ORCID logo ,
Eleonora Čapelja
ORCID logo ,
Maja Karaman
ORCID logo
Version 1 : Received: 29 November 2023 / Approved: 30 November 2023 / Online: 30 November 2023 (10:38:54 CET)

A peer-reviewed article of this Preprint also exists.

Rašeta, M.; Kebert, M.; Mišković, J.; Rakić, M.; Kostić, S.; Čapelja, E.; Karaman, M. Polyamines in Edible and Medicinal Fungi from Serbia: A Novel Perspective on Neuroprotective Properties. J. Fungi 2024, 10, 21. Rašeta, M.; Kebert, M.; Mišković, J.; Rakić, M.; Kostić, S.; Čapelja, E.; Karaman, M. Polyamines in Edible and Medicinal Fungi from Serbia: A Novel Perspective on Neuroprotective Properties. J. Fungi 2024, 10, 21.

Abstract

The therapeutic effectiveness of current neurodegenerative diseases treatments is still under debate because of problems with bioavailability and a range of side effects. Fungi, which are increasingly recognized as sources of natural antioxidants and AChE enzyme inhibitors, may thus serve as potent neuroprotective agents. Previous studies have associated the anti-AChE and antioxidant activities of fungi mostly with polysaccharides and phenolic compounds, while other secondary metabolites such as polyamines (PAs) have been neglected. This study aimed to investigate eight edible and medicinal fungi from Serbia, marking the initial investigation into the neuroprotective capabilities of Postia caesia, Clitocybe odora, Clitopilus prunulus, and Morchella elata. Neuroprotective activity was examined using Ellman assay, while antioxidant capacity was tested by conducting DPPH, NO, ABTS and FRAP tests. PAs levels were determined by HPLC coupled with fluorescent detection. Ganoderma applanatum and Lepista nuda exhibited the most robust anti-AChE (98.05 ± 0.83% and 99.94 ± 3.10%, respectively) and antioxidant activities attributed to the synergistic effects of total protein, total phenolic and PAs levels. Furthermore, P. caesia displayed significant AChE inhibition (88.21 ± 4.76%), primarily linked to elevated SPD (62.98 ± 3.19%) and PUT levels (55.87 ± 3.16%). Our results highlight the need of thorough research to comprehend the intricate relationships between distinct fungus species and AChE inhibition. However, it's important to recognize that more research is required to identify the precise substances causing the reported inhibitory effects.

Keywords

polyamines; G. applanatum; L. nuda; antioxidant; phenolics; synergism; anti-acetylcholinesterase

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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