Version 1
: Received: 23 November 2023 / Approved: 23 November 2023 / Online: 24 November 2023 (02:39:23 CET)
How to cite:
Singh, Y.; Kaur, B.; Kaur, M.; HM, Y.; Mukhopadhyay, C. Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in Popular Dog Breeds in India. Preprints2023, 2023111536. https://doi.org/10.20944/preprints202311.1536.v1
Singh, Y.; Kaur, B.; Kaur, M.; HM, Y.; Mukhopadhyay, C. Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in Popular Dog Breeds in India. Preprints 2023, 2023111536. https://doi.org/10.20944/preprints202311.1536.v1
Singh, Y.; Kaur, B.; Kaur, M.; HM, Y.; Mukhopadhyay, C. Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in Popular Dog Breeds in India. Preprints2023, 2023111536. https://doi.org/10.20944/preprints202311.1536.v1
APA Style
Singh, Y., Kaur, B., Kaur, M., HM, Y., & Mukhopadhyay, C. (2023). Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in Popular Dog Breeds in India. Preprints. https://doi.org/10.20944/preprints202311.1536.v1
Chicago/Turabian Style
Singh, Y., Yatish HM and Chandra Mukhopadhyay. 2023 "Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in Popular Dog Breeds in India" Preprints. https://doi.org/10.20944/preprints202311.1536.v1
Abstract
For the parentage testing in canine microsatellite length polymorphism markers were used to check the efficacy of the markers. In the current study 5’ fluorescently labeled 12 SSR markers were used to check the use of the markers in popular owned-dog breeds (Labrador, German Shepherd, Pug, Mudhol Hound, Tibetan Mastiff, Gaddi dog, Beagle, Belgian Malinois, Pointer, and Cane Corso) maintained of India (not necessarily indigenous breeds). The number of alleles, heterozygosity, polymorphism information content, and probability of exclusion were determined for all the markers to check the effectiveness of the markers. The mean number of alleles per locus ranged from 5 to 29 and the effective number of alleles ranged from 3.6 to 15.2. The expected heterozygosity was greater than 0.73. The population inbreeding coefficient (FIS) demonstrated that there was no inbreeding in the breeds studied, as the samples were collected from owners and dog breeders belonging to various states, including Punjab, Haryana, Himachal Pradesh, and Karnataka. The polymorphism information content and the probability of the exclusion values were greater than 0.65. the combined probability of exclusion for all the breeds was (2.82E-12) 0.99999995. The results indicated that the selected 12 markers are effective enough to determine the parentage of the dogs.
Keywords
Canine; microsatellite; parentage determination; probability of exclusion
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
