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Recombinant Human Peptide Growth Factors rhBMP7 (Bone Morphogenetic Protein 7) and rhPDGF-BB (Platelet-Derived Growth Factor BB) for Osteoporosis Treatment in an Oophorectomized Rat Model
Reis, T.G.; Del Colletto, A.M.S.; Silva, L.A.S.; Koga, B.A.A.; Sogayar, M.C.; Carreira, A.C.O. Recombinant Human Peptide Growth Factors, Bone Morphogenetic Protein-7 (rhBMP7), and Platelet-Derived Growth Factor-BB (rhPDGF-BB) for Osteoporosis Treatment in an Oophorectomized Rat Model. Biomolecules2024, 14, 317.
Reis, T.G.; Del Colletto, A.M.S.; Silva, L.A.S.; Koga, B.A.A.; Sogayar, M.C.; Carreira, A.C.O. Recombinant Human Peptide Growth Factors, Bone Morphogenetic Protein-7 (rhBMP7), and Platelet-Derived Growth Factor-BB (rhPDGF-BB) for Osteoporosis Treatment in an Oophorectomized Rat Model. Biomolecules 2024, 14, 317.
Reis, T.G.; Del Colletto, A.M.S.; Silva, L.A.S.; Koga, B.A.A.; Sogayar, M.C.; Carreira, A.C.O. Recombinant Human Peptide Growth Factors, Bone Morphogenetic Protein-7 (rhBMP7), and Platelet-Derived Growth Factor-BB (rhPDGF-BB) for Osteoporosis Treatment in an Oophorectomized Rat Model. Biomolecules2024, 14, 317.
Reis, T.G.; Del Colletto, A.M.S.; Silva, L.A.S.; Koga, B.A.A.; Sogayar, M.C.; Carreira, A.C.O. Recombinant Human Peptide Growth Factors, Bone Morphogenetic Protein-7 (rhBMP7), and Platelet-Derived Growth Factor-BB (rhPDGF-BB) for Osteoporosis Treatment in an Oophorectomized Rat Model. Biomolecules 2024, 14, 317.
Abstract
Bone morphogenetic protein (BMP) and platelet-derived growth factor (PDGF) are known to regulate and stimulate osteogenesis, both proteins playing an important role in bone homeostasis, constituting good candidates for osteoporosis´ treatment. Herein we aim to evaluate the effects of recombinant human BMP-7 (rhBMP7) and PDGF-BB (rhPDGF-BB) in oophorectomy-induced osteoporosis in rats. To this end, 40 Sprague-Dawley rats were submitted to oophorectomy surgery and treatments were started at the 100th day after the surgery, when all animals showed signs of osteoporosis. These peptide growth factors were applied intravenously once or twice a week and animals were followed for a total of five weeks. Two weeks after the end of the treatments, the animals were euthanized, the tissues were collected and Alkaline Phosphatase, X-ray, micro-CT and histology were assessed. The results indicate that the most promising treatments are: 20 µg/kg PDGF-BB + 30 µg/kg BMP-7 2x/week and 30 µg/kg BMP-7 2x/week, presenting an increase of 15% (p<0.05) and 13% (p<0.05) in bone volume fraction and 21% (p<0.05) and 23% (p<0.05) in trabecular number, respectively. In conclusion, rhPDGF-BB and rhBMP-7 are capable of inducing an increase in bone volume and density in this osteoporotic animal model, constituting interesting candidates for osteoporosis treatment.
Keywords
Osteoporosis; BMP-7; PDGF-BB; peptide growth factors; bone repair.
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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