Alan, Y.; Yılmaz, N.; Cabuk, M. Inula helenium subsp.Pseudohelenium: Phenolic Content, In Vitro Antimicrobial, Antioxidant, Antiproliferative, and DNA Protective Activities. Preprints2023, 2023110484. https://doi.org/10.20944/preprints202311.0484.v1
APA Style
Alan, Y., Yılmaz, N., & Cabuk, M. (2023). <em>Inula helenium subsp.</em> <em>Pseudohelenium</em>: Phenolic Content, In Vitro Antimicrobial, Antioxidant, Antiproliferative, and DNA Protective Activities. Preprints. https://doi.org/10.20944/preprints202311.0484.v1
Chicago/Turabian Style
Alan, Y., Nimet Yılmaz and Mehmet Cabuk. 2023 "<em>Inula helenium subsp.</em> <em>Pseudohelenium</em>: Phenolic Content, In Vitro Antimicrobial, Antioxidant, Antiproliferative, and DNA Protective Activities" Preprints. https://doi.org/10.20944/preprints202311.0484.v1
Abstract
Plants have been used in alternative medicine for centuries in the treatment of many diseases with the metabolites they contain. The goal of this work was to investigate the phenolic profile, antioxidant capacity and biological activity of I. helenium subsp. pseudohelenium extracts. Phe-nolic (HPLC) substances in leaf ethanol (L-EtOH), leaf pure water (L-PW), root ethanol (R-EtOH), and root pure water (R-PW) extracts of Inula helenium subsp. pseudohelenium plant were investi-gated. Also, in vitro the antimicrobial (hollow agar), antioxidant (DPPH, ABTS.+, FRAP, CU-PRAC), antiproliferative (MTT assay against HT-29, MCF-7 and L-929), and DNA (Agarose gel electrophoresis) protective activities of the samples were examined. Although gallic acid was not detected in the extracts, the presence of curcumin was detected only in EtOH extracts. L-EtOH exhibited the best antimicrobial activity. According to the antioxidant results, it was found that the L-EtOH extract had an activity profile close to the standard antioxidants in general. It was also found that the L-EtOH extract stabilized the DNA by disrupting the scavenging effect of H2O2+DMSO. According to the antiproliferative activity results, EtOH extracts exhibited the strongest effect, especially against MCF7. When evaluated in general terms, it was found that L-EtOH extract showed better activity than other extracts. It is important to conduct more in vivo and in vitro studies after isolating the active components of extracts e.g. L-EtOH, which have good effects in terms of biological activity.
Chemistry and Materials Science, Medicinal Chemistry
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