Version 1
: Received: 4 November 2023 / Approved: 6 November 2023 / Online: 6 November 2023 (10:33:41 CET)
How to cite:
Tang, L.; Zheng, Y.; Wang, H.; Khan, I.; Xie, W. Tissue-Specific Transcriptomic Analysis Reveals the Response Mechanism of Cold Stress in Poa crymophila, a Native Grass in Qinghai-Tibet Plateau. Preprints2023, 2023110336. https://doi.org/10.20944/preprints202311.0336.v1
Tang, L.; Zheng, Y.; Wang, H.; Khan, I.; Xie, W. Tissue-Specific Transcriptomic Analysis Reveals the Response Mechanism of Cold Stress in Poa crymophila, a Native Grass in Qinghai-Tibet Plateau. Preprints 2023, 2023110336. https://doi.org/10.20944/preprints202311.0336.v1
Tang, L.; Zheng, Y.; Wang, H.; Khan, I.; Xie, W. Tissue-Specific Transcriptomic Analysis Reveals the Response Mechanism of Cold Stress in Poa crymophila, a Native Grass in Qinghai-Tibet Plateau. Preprints2023, 2023110336. https://doi.org/10.20944/preprints202311.0336.v1
APA Style
Tang, L., Zheng, Y., Wang, H., Khan, I., & Xie, W. (2023). Tissue-Specific Transcriptomic Analysis Reveals the Response Mechanism of Cold Stress in Poa crymophila, a Native Grass in Qinghai-Tibet Plateau. Preprints. https://doi.org/10.20944/preprints202311.0336.v1
Chicago/Turabian Style
Tang, L., Imran Khan and Wengang Xie. 2023 "Tissue-Specific Transcriptomic Analysis Reveals the Response Mechanism of Cold Stress in Poa crymophila, a Native Grass in Qinghai-Tibet Plateau" Preprints. https://doi.org/10.20944/preprints202311.0336.v1
Abstract
Poa crymophila is a perennial, cold-tolerant, native grass species, widely distributed to the Qing-hai-Tibet Plateau. However, the molecular mechanism behind the cold stress tolerance and role of key regulatory genes and pathways of P. crymophila are poorly understood yet. Therefore, the present study investigated the physiological and transcriptome responses of P. crymophila’s roots, stems, and leaves under cold stress to explore the molecular mechanism of cold tolerance. Results of the present study suggested that cold stress significantly changed the physiologic characteristics of roots, stems, and leaves of P. crymophila. In addition, the transcriptome results showed that 4434, 8793, and 14942 differentially expressed genes (DEGs) were identified in roots, stems, and leaves, respectively; however, 464 DEGs were commonly identified in these three tissues. The Gene On-tology (GO) results showed that a large numbers of DEGs were significantly enriched in the pho-tosynthetic related categories in leaves. In addition, the “response to stimulus” category was sig-nificantly enriched in roots and stems. The Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that DEGs involved in “phenylpropanoid biosynthesis” were significantly enriched in roots, “photosynthesis” and “circadian rhythm-plant” pathways significantly enriched in stems and leaves, starch and sucrose metabolism, and galactose metabolism were pathways significantly enriched in three tissues. Weighted gene coexpression network analysis (WGCNA) identified Hub genes involved in P. crymophila cold response. This study provides new insights into the molecular mechanisms underlying the cold tolerance of P. crymophila belowground and aboveground tissues. In addition, specific genes involved in Ca2+ signaling, ROS scavenging system, hormones, circadian clock, photosynthesis, and transcription factors (TFs) were identified in P. crymophila. The identi-fication of key genes may provide valuable resources for further functional genomic and breeding studies.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
