Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Virome of the tabanids from Russia

Alexander G. Litov
,
Oxana A. Belova
ORCID logo ,
Ivan S. Kholodilov
ORCID logo ,
Anna S. Kalyanova
,
Magomed N. Gadzhikurbanov
ORCID logo ,
Anastasia A. Rogova
,
Larissa V. Gmyl
,
Galina G. Karganova
*
Version 1 : Received: 30 October 2023 / Approved: 30 October 2023 / Online: 31 October 2023 (13:01:42 CET)

A peer-reviewed article of this Preprint also exists.

Litov, A.G.; Belova, O.A.; Kholodilov, I.S.; Kalyanova, A.S.; Gadzhikurbanov, M.N.; Rogova, A.A.; Gmyl, L.V.; Karganova, G.G. Viromes of Tabanids from Russia. Viruses 2023, 15, 2368. Litov, A.G.; Belova, O.A.; Kholodilov, I.S.; Kalyanova, A.S.; Gadzhikurbanov, M.N.; Rogova, A.A.; Gmyl, L.V.; Karganova, G.G. Viromes of Tabanids from Russia. Viruses 2023, 15, 2368.

Abstract

Advances in sequencing technologies and bioinformatics have greatly enhanced our knowledge of virus biodiversity. Currently, viromes of haematophagous invertebrates, such as mosquitoes and ixodid ticks, are actively studied. Tabanidae (Diptera) is a widespread family, with members mostly known for their persistent haematophagous behavior. They transmit viral, bacterial and other pathogens, both biologically and mechanically. However, tabanid viromes remain severely understudied. In this study, we used high-throughput sequencing to describe the viromes of several species of Hybomitra, Tabanus, Chrysops, and Haematopota genera, which were collected in Russia. We assembled 14 full coding genomes of the novel viruses, 4 partial coding genomes, as well as several fragmented viral sequences, which presumably belong to another 12 new viruses. All the discovered viruses were tested on their ability to replicate in the mammalian porcine embryo kidney (PEK), tick HAE/CTVM8, and mosquito C6/36 cell lines. In total, 16 viruses were detected in at least one cell culture after three passages (for PEK and C6/36) or 3 weeks of persistence in HAE/CTVM8. However, in the majority of cases, qPCR showed a decline in virus load over time. Nevertheless, four viruses were detected after six passages.

Keywords

Tabanidae; Narnaviridae; Totiviridae; Flaviviridae; Xinmoviridae; Permutotetraviridae; Negevirus; Rhabdoviridae; Solemoviridae; Picornavirales

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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