Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Comprehensive Analysis of miRNA and mRNA Expression Profiles during Muscle Development of the Longest Dorsal Muscle in Gannan Yaks and Jeryaks

Dashan Guo
,
Yali Wei
,
Xupeng Li
,
Yanbin Bai
,
Zhanxin Liu
,
Jingsheng Li
,
Zongchang Chen
,
Bingang Shi
,
Zhidong Zhao
* ORCID logo ,
Jiang Hu
* ,
Xiangmin Han
,
Jiqing Wang
ORCID logo ,
Xiu Liu
,
Shaobin Li
,
Fangfang Zhao
Version 1 : Received: 19 October 2023 / Approved: 19 October 2023 / Online: 23 October 2023 (11:05:29 CEST)

How to cite: Guo, D.; Wei, Y.; Li, X.; Bai, Y.; Liu, Z.; Li, J.; Chen, Z.; Shi, B.; Zhao, Z.; Hu, J.; Han, X.; Wang, J.; Liu, X.; Li, S.; Zhao, F. Comprehensive Analysis of miRNA and mRNA Expression Profiles during Muscle Development of the Longest Dorsal Muscle in Gannan Yaks and Jeryaks. Preprints 2023, 2023101377. https://doi.org/10.20944/preprints202310.1377.v1 Guo, D.; Wei, Y.; Li, X.; Bai, Y.; Liu, Z.; Li, J.; Chen, Z.; Shi, B.; Zhao, Z.; Hu, J.; Han, X.; Wang, J.; Liu, X.; Li, S.; Zhao, F. Comprehensive Analysis of miRNA and mRNA Expression Profiles during Muscle Development of the Longest Dorsal Muscle in Gannan Yaks and Jeryaks. Preprints 2023, 2023101377. https://doi.org/10.20944/preprints202310.1377.v1

Abstract

The Jeryak, a hybrid offspring of Gannan yak and Jersey cattle, exhibits evident hybrid advantages over the Gannan yak in terms of production performance and other various aspects. The small non-coding RNAs known as miRNAs exert a significant regulatory influence on gene expression post-transcriptionally. However, the regulatory mechanism of miRNA associated with muscle development in Jeryak remains elusive. To elucidate the regulatory role of miRNAs in orches-trating the skeletal muscle development in Jeryak, we selected dorsal longissimus muscle tissues from Gannan yak and Jeryak for transcriptome sequencing analysis. A total of 230 (DE)miRNAs and 1819 differentially expressed mRNAs (DEMs) were identified in the longest dorsal muscle of Gannan yak and Jeryak. The functional enrichment analysis revealed a significantly enrichment of target genes from differentially expressed (DE)miRNAs and DEMs in signaling pathways associ-ated with muscle growth, such as the Ras signaling pathway and the MAPK signaling pathway. The network of interactions between miRNA and mRNA suggested that some differentially ex-pressed miRNAs, including miR-2478-z, miR-339-x, novel-m0036-3p and novel-m0037-3p, played a pivotal role in facilitating muscle development. These findings help us to deepen our under-standing of the hybrid dominance of Jeryaks and provide a theoretical basis for further research on the regulatory mechanisms of miRNAs associated with Jeryak muscle growth and development.

Keywords

Jeryak; longissimus dorsi; RNA-seq; miRNA; production performance

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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