Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

SOX2 and SOX9 Expression in Developing Postnatal Opossum (Monodelphis domestica) Cortex

Zrinko Baričević
ORCID logo ,
Marta Pongrac
,
Matea Ivaničić
,
Helena Hreščak
,
Ivana Tomljanović
,
Antonela Petrović
,
Dan Cojoc
ORCID logo ,
Miranda Mladinic
ORCID logo ,
Jelena Ban
* ORCID logo
Version 1 : Received: 12 September 2023 / Approved: 13 September 2023 / Online: 13 September 2023 (07:56:25 CEST)

A peer-reviewed article of this Preprint also exists.

Baričević, Z.; Pongrac, M.; Ivaničić, M.; Hreščak, H.; Tomljanović, I.; Petrović, A.; Cojoc, D.; Mladinic, M.; Ban, J. SOX2 and SOX9 Expression in Developing Postnatal Opossum (Monodelphis domestica) Cortex. Biomolecules 2024, 14, 70. https://doi.org/10.3390/biom14010070 Baričević, Z.; Pongrac, M.; Ivaničić, M.; Hreščak, H.; Tomljanović, I.; Petrović, A.; Cojoc, D.; Mladinic, M.; Ban, J. SOX2 and SOX9 Expression in Developing Postnatal Opossum (Monodelphis domestica) Cortex. Biomolecules 2024, 14, 70. https://doi.org/10.3390/biom14010070

Abstract

(1) Background: Central nervous system (CNS) development is characterized by dynamic changes in cell proliferation and differentiation. Key regulators of these transitions are the transcription factors such as SOX2 and SOX9. SOX2 is involved in the maintenance of progenitor cell state and neural stem cell multipotency, while SOX9, expressed in neurogenic niches, plays an important role in neuron/glia switch with predominant expression in astrocytes in the adult brain. (2) Methods: To validate SOX2 and SOX9 expression pattern in dissociated primary neuronal cultures derived from opossum (Monodelphis domestica), we used immunohistochemistry (IHC) and the isotropic fractionator method on fixed cortical tissue from comparable postnatal ages; (3) Results: Neurons positive for both neuronal (NeuN or TUJ1) and stem cell (SOX2) markers were identified and their presence was confirmed with all methods and postnatal age groups (P4-6, P6-18 and P30) analyzed. SOX9 showed exclusive staining in non-neuronal cells, and it was coexpressed with SOX2. (4) Conclusions: The persistence of SOX2 expression in developing cortical neurons of M. domestica during first post-natal month implies the functional role of SOX2 during neuronal differentiation and maturation, which was not previously reported in opossums.

Keywords

opossum; cortex; development; neurogenesis; primary neuronal cultures; isotropic fractionator; SOX2; SOX9

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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