Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation

Giorgia Innamorati; Marina Sanchez-Petidier; Giulia Bergafora; Camilla Codazzi; Valentina Palma; Francesca Camera; Caterina Merla; Franck M André; Maria Pedraza; Victoria Moreno Manzano; Laura Caramazza; Micol Colella; Paolo Marracino; Claudia Consales

doi:10.20944/preprints202309.0637.v1

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preprints.org > biology and life sciences > biology and biotechnology > doi: 10.20944/preprints202309.0637.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation

Giorgia Innamorati

Marina Sanchez-Petidier

Victoria Moreno Manzano

Version 1 : Received: 8 September 2023 / Approved: 11 September 2023 / Online: 11 September 2023 (07:30:21 CEST)

How to cite: Innamorati, G.; Sanchez-Petidier, M.; Bergafora, G.; Codazzi, C.; Palma, V.; Camera, F.; Merla, C.; André, F.M.; Pedraza, M.; Moreno Manzano, V.; Caramazza, L.; Colella, M.; Marracino, P.; Consales, C. Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation. Preprints 2023, 2023090637. https://doi.org/10.20944/preprints202309.0637.v1 Innamorati, G.; Sanchez-Petidier, M.; Bergafora, G.; Codazzi, C.; Palma, V.; Camera, F.; Merla, C.; André, F.M.; Pedraza, M.; Moreno Manzano, V.; Caramazza, L.; Colella, M.; Marracino, P.; Consales, C. Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation. Preprints 2023, 2023090637. https://doi.org/10.20944/preprints202309.0637.v1

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MDPI and ACS Style

Innamorati, G.; Sanchez-Petidier, M.; Bergafora, G.; Codazzi, C.; Palma, V.; Camera, F.; Merla, C.; André, F.M.; Pedraza, M.; Moreno Manzano, V.; Caramazza, L.; Colella, M.; Marracino, P.; Consales, C. Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation. Preprints 2023, 2023090637. https://doi.org/10.20944/preprints202309.0637.v1

APA Style

Innamorati, G., Sanchez-Petidier, M., Bergafora, G., Codazzi, C., Palma, V., Camera, F., Merla, C., André, F.M., Pedraza, M., Moreno Manzano, V., Caramazza, L., Colella, M., Marracino, P., & Consales, C. (2023). Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation. Preprints. https://doi.org/10.20944/preprints202309.0637.v1

Chicago/Turabian Style

Innamorati, G., Paolo Marracino and Claudia Consales. 2023 "Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation" Preprints. https://doi.org/10.20944/preprints202309.0637.v1

Abstract

In the tissue regeneration field, stem cell transplantation and the control of their differentiation represent a promising therapeutic strategy. The second aspect can be achieved using electrical stimulation. This study aims to characterize the effect of a microsecond electrical stimulation on MSCs and iNSCs, in the context of the RISEUP FET-OPEN project (n. 964562) that seeks to control these cells differentiation for the spinal cord injury treatments. Here, the effect of a specific microsecond electric pulses stimulation, characterized by bipolar pulses of 100 µs + 100 µs, delivered for 30 minutes at an intensity of 250 V/cm, on i) cell proliferation, ii) cell cycle, iii) gene expression and iv) apoptosis was evaluated. Results show that the stimulation does not affect cell proliferation, cell cycle, and apoptosis, but induced some variations in gene expression, in particular in EGR1, FOS, and POU5F1. These ob-servations led us to deeply investigate the cell proliferation until 72h from the stimulation observing an increase in the iNSCs. The main outcome of this study is that the chosen stimulation protocol is safe and not toxic for MSCs and iNSCs. The observed variations in the gene expression need to be deeply investigated to assess the molecular mechanisms involved.

Keywords

stem cells; microsecond electric pulses stimulation; cell proliferation; gene expression

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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