Taofeek, O.O., Oyewole, O.M., Sulaimon, L.A. et al. Abrus precatorius leaf bioactives: invivo anti-diabetes mellitus type-1 activity, PPARA and SCD as novel targets. J.Umm Al-Qura Univ. Appll. Sci. (2024). https://doi.org/10.1007/s43994-023-00113-x
Taofeek, O.O., Oyewole, O.M., Sulaimon, L.A. et al. Abrus precatorius leaf bioactives: invivo anti-diabetes mellitus type-1 activity, PPARA and SCD as novel targets. J.Umm Al-Qura Univ. Appll. Sci. (2024). https://doi.org/10.1007/s43994-023-00113-x
Taofeek, O.O., Oyewole, O.M., Sulaimon, L.A. et al. Abrus precatorius leaf bioactives: invivo anti-diabetes mellitus type-1 activity, PPARA and SCD as novel targets. J.Umm Al-Qura Univ. Appll. Sci. (2024). https://doi.org/10.1007/s43994-023-00113-x
Taofeek, O.O., Oyewole, O.M., Sulaimon, L.A. et al. Abrus precatorius leaf bioactives: invivo anti-diabetes mellitus type-1 activity, PPARA and SCD as novel targets. J.Umm Al-Qura Univ. Appll. Sci. (2024). https://doi.org/10.1007/s43994-023-00113-x
Abstract
ObjectivesAbrus precatorius (AP) is a medicinal plant seldomly studied for its beneficial effects and as such significant gap exists in understanding the basis by which AP leaf bioactives (APLBs) elicit beneficial effects against diabetes mellitus (DM) type-1. MethodsWe estimated DM type-1 related parameters - total protein (TP), direct bilirubin (DB), urea, creatinine, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and serum glucose (SG) after treatment with methanol extracts of AP leaves (APMLE) for 21 days followed by histopathological analysis of kidney and liver sections. APLBs were collected from GCMS fractions, database and literature searches and common targets were cross-intersected with annotated DM type-1 genes from experimental GSE14503 microarray dataset and genecard database. Overlapping differentially expressed genes were collected, their protein-protein interaction network was constructed and analyzed using various bioinformatics tools: Enrichr, SRplot, GSEA, Cytoscape, PyRx, and Discovery Studio to provide insight into the potential molecular basis of APLBs in DM-type-1.Results 15 compounds were identified from GCMS analysis of APMLE.Antidiabetic potential of APMLE was observed with significant (p <0.05) normalization of SG, TP, DB, ALT, AST, ALP, urea & creatinine while hepatorenal photomicrographs indicated moderate safety of use. Erucic acid, oleic acid, phytol and stigmasterol interact with 25 type-1 DM biomarkers revealed to be enriched in lipid and prostaglandin metabolic processes, neuroactive ligand receptor interaction, PPAR signaling pathway, diabetic cardiomyopathy, and cAMP signaling pathway. Furthermore, PPARalpha (peroxisome proliferator-activated alpha) and SCD (stearoyl-coenzyme A desaturase) were revealed as core biotargets interacting with APLBs via hydrogen bond, hydrophobic interaction and van der Waals forces from docking study. ConclusionThis study demonstrated the potency of APLBs in vivo against type-1 DM. The potential basis of action has been studied from computational perspective. Future interests may provide additional experimental data into mechanisms by which APLBs elicit this remarkable ability.
Keywords
Abrus precatorius; diabetes; GSEA; PPARalpha; SCD
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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