Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth

Yuka Miwa
,
Mahanama Geegana Gamage Awanthi
ORCID logo ,
Kouichi Soga
ORCID logo ,
Atsuko Tanaka
,
Michihiro Ito
,
Yuichiro Numata
,
Yoichi Sato
,
Teruko Konishi
* ORCID logo
Version 1 : Received: 15 August 2023 / Approved: 16 August 2023 / Online: 16 August 2023 (10:10:17 CEST)

A peer-reviewed article of this Preprint also exists.

Miwa, Y.; Awanthi, M.G.G.; Soga, K.; Tanaka, A.; Ito, M.; Numata, Y.; Sato, Y.; Konishi, T. The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth. Plants 2023, 12, 3274. Miwa, Y.; Awanthi, M.G.G.; Soga, K.; Tanaka, A.; Ito, M.; Numata, Y.; Sato, Y.; Konishi, T. The Cell Wall Characterization of Brown Alga Cladosiphon okamuranus during Growth. Plants 2023, 12, 3274.

Abstract

The present study provides new insights into the growth of brown algal cell wall by showing that cell wall polysaccharide plays an important role in the process of growth consider- ing the physicochemical characteristic of young and old Cladosiphon okamuranus. To determine its structural variation in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC–I), HC-II, and cellulose, and analyzed physicochemically. Results showed that almost 80% of the total recovery cell wall from both young and old thalli was HW and HC-I contained mainly fucoidan composed of Fuc, GlcA, and sulfate in molar ratios of 1.0: 0.3: 0.6~0.7 and 1.0: 0.3: 0.2~0.3, respectively. Fucoidan in HW was a highly sulfated matrix polysaccharide abundance in young thalli, while fucoidan in HC-I was rich in old thalli and functions as hemicellulose in land plants, crosslink with cellulose and strengthen the cell wall. We found that HW and HC-I were particularly involved in the growth and strength of old thalli appeared to be due to the deposition of HC-I and reducing of water content during the growth process.

Keywords

Cladosiphon okamuranus; Okinawa Mozuku; Cell wall polysaccharide; Fucoidan; Growth

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.