Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Transgenic Cotton Plants Overexpressing GhKAR or GhENR Exhibit Increased Seed Oil Content

Li Liu
,
Dan Wang
,
Jinping Hua
ORCID logo ,
Xianhui Kong
,
Xuwen Wang
,
Juan Wang
,
Aijun Si
,
Fuxiang Zhao
,
Wenhao Liu
,
Yu Yu
,
Zhiwen Chen
*
Version 1 : Received: 4 August 2023 / Approved: 7 August 2023 / Online: 7 August 2023 (11:55:59 CEST)

A peer-reviewed article of this Preprint also exists.

Liu, L.; Wang, D.; Hua, J.; Kong, X.; Wang, X.; Wang, J.; Si, A.; Zhao, F.; Liu, W.; Yu, Y.; Chen, Z. Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars. Plants 2023, 12, 3437. Liu, L.; Wang, D.; Hua, J.; Kong, X.; Wang, X.; Wang, J.; Si, A.; Zhao, F.; Liu, W.; Yu, Y.; Chen, Z. Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars. Plants 2023, 12, 3437.

Abstract

Three carbon-chain extension genes associated with fatty acid synthesis in cotton, namely GhKAR, GhHAD, and GhENR, play important roles in oil accumulation in cotton seeds. In the present study, these three genes were cloned and characterized. The expression patterns of GhKAR, GhHAD and GhENR in the high seed oil content cultivars 10H1007, 10H1014, and 10H1041 differed somewhat compared with those of 2074B with low seed oil content at different stages of seed development. GhKAR showed all three cultivars showed higher transcript levels than that of 2074B at 10, 40 and 45 DPA. The expression pattern of GhHAD showed a lower transcript level than that of 2074B at both 10 and 30 DPA but higher transcript level than that of 2074B at 40 DPA. GhENR showed a lower transcript level than that of 2074B at both 15 and 30 DPA. The highest transcript levels of GhKAR and GhENR were detected at 15 DPA in 10H1007, 10H1014, and 10H1041 compared with 2074B. From 5 to 45 days post anthesis (DPA) cotton seed, the oil content accumulated continuously in the developing seed. Oil accumulation reached the peak at 40 DPA to 45 DPA and slightly decreased in mature seed. In addition, GhKAR and GhENR showed different expression patterns in fiber and ovule development processes, in which they showed high expression levels at 20 DPA during fiber elongation stage, but their expression level peaked at 15 DPA during ovule development processes. These two genes showed the lowest expression levels at the late seed maturation stage, while GhHAD showed peak at 10 DPA of fiber development. Compared to 2074B, the oil contents of GhKAR and GhENR overexpression lines increased 1.05~1.08 folds. These results indicated that GhHAD, GhENR and GhKAR were involved in both seed oil synthesis and fiber elongation with dual biological functions in cotton.

Keywords

fatty acid; seed oil content; fiber development; gene expression; upland cotton (Gossypium hirsutum)

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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