Wan, W.; Zhao, H.; Yu, K.; Xiang, Y.; Dai, W.; Du, C.; Tian, E. Exploration into Natural Variation Genes Associated with Determinate and Capitulum-like Inflorescence in Brassica napus. Int. J. Mol. Sci.2023, 24, 12902.
Wan, W.; Zhao, H.; Yu, K.; Xiang, Y.; Dai, W.; Du, C.; Tian, E. Exploration into Natural Variation Genes Associated with Determinate and Capitulum-like Inflorescence in Brassica napus. Int. J. Mol. Sci. 2023, 24, 12902.
Wan, W.; Zhao, H.; Yu, K.; Xiang, Y.; Dai, W.; Du, C.; Tian, E. Exploration into Natural Variation Genes Associated with Determinate and Capitulum-like Inflorescence in Brassica napus. Int. J. Mol. Sci.2023, 24, 12902.
Wan, W.; Zhao, H.; Yu, K.; Xiang, Y.; Dai, W.; Du, C.; Tian, E. Exploration into Natural Variation Genes Associated with Determinate and Capitulum-like Inflorescence in Brassica napus. Int. J. Mol. Sci. 2023, 24, 12902.
Abstract
Brassica napus is an important vegetable and oil crop worldwide. The research is meaningful for yield and plant architecture in B. napus. In this study, one natural mutant (MT) line with determinate and capitulum-like inflorescence was chosen for further study. The paraffin sectioning detected that the inflorescence apex began to split and developed into one terminal flower, starting from five-leaf stage. Genetic analysis indicated that the segregation patterns of inflorescences in the F2 populations supported a digenic inheritance model, which was further approved by BSA-Seq technique. The BSA-Seq method detected two QTL region on C02 (14.27-18.41 Mb) and C06 (32.98-33.68 Mb) for the genetic control of determinate inflorescences in MT plants. In addition, the expression profile in MT compared with WT was analysized, and a total of 133 candidate genes for regulating the flower development (75 genes, 56.4%), shoot meristem development (29 genes, 21.8%) and inflorescence meristem development (13 genes, 9.8%) were identified. Then one joint analysis combing BSA-Seq and RNA-Seq identified two candidate gene of BncTFL1 and BncAP1 for regulating the MT phenotype. Besides, the potential utilization of the MT plants was also discussed.
Keywords
Brassica napus; Determinate and capitulum-like inflorescence; terminal flower; BncAP1; BncTFL1
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
