Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Fermentation Patterns, Methane Production and Microbial Population Under in Vitro Conditions From Two Unconventional Feed Resources Incorporated in Ruminant Diets

Version 1 : Received: 20 July 2023 / Approved: 21 July 2023 / Online: 25 July 2023 (10:10:29 CEST)

A peer-reviewed article of this Preprint also exists.

Araiza Ponce, K.A.; Gurrola Reyes, J.N.; Martínez Estrada, S.C.; Salas Pacheco, J.M.; Palacios Torres, J.; Murillo Ortiz, M. Fermentation Patterns, Methane Production and Microbial Population under In Vitro Conditions from Two Unconventional Feed Resources Incorporated in Ruminant Diets. Animals 2023, 13, 2940. Araiza Ponce, K.A.; Gurrola Reyes, J.N.; Martínez Estrada, S.C.; Salas Pacheco, J.M.; Palacios Torres, J.; Murillo Ortiz, M. Fermentation Patterns, Methane Production and Microbial Population under In Vitro Conditions from Two Unconventional Feed Resources Incorporated in Ruminant Diets. Animals 2023, 13, 2940.

Abstract

In this study, four experimental treatments were evaluated: (T1) alfalfa hay + concentrate, (50:50%, DM); (T2) alfalfa hay + Leucaena leucocephala + concentrate, (30:20:50%, DM); (T3) alfalfa hay + prickly pear + concentrate, (30:20:50%, DM); and (T4) alfalfa hay + Leucaena leucocephala + prickly pear + concentrate, (30:10:10:50%, DM). NH3-N concentrations in T2 and T4 decreased when replaced alfalfa hay in 20 and 10 %, respectively. Treatments did not affect the concentration of total volatile fatty acids (TVFA) between T3 and T4 (p>0.05); while the concentrations among T1 and T2 were different (P<0.05). T2 showed a reduction of 25.5 % in the methane production when compared to T1(p < 0.05).The lowest concentrations of protozoa were observed in T2 and T4, which contained Leucaena leucocephala (T2) and Leucaena leucocephala + prickly pear (T4) (p<0.05). The highest concentration of total methanogens was recorded in T1 and were different to T2, T3 and T4 (p<0.05). Leucaena leucocephala at a inclusion percentage of 20 % decreased the methane when compared to T1; whereas prickly pear there was not a positive effect the methane production.

Keywords

methane; ammonia; Leucaena leucocephala; Pricky pear; microbial population; in vitro fermentation

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

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