Lee, J.; Hong, J.; Lee, J.; Lee, C.; Kim, T.; Jeong, Y.; Kim, K.; Jung, I. Precise Filtration of Chronic Myeloid Leukemia Cells by an Ultrathin Microporous Membrane with Backflushing to Minimize Fouling. Membranes2023, 13, 707.
Lee, J.; Hong, J.; Lee, J.; Lee, C.; Kim, T.; Jeong, Y.; Kim, K.; Jung, I. Precise Filtration of Chronic Myeloid Leukemia Cells by an Ultrathin Microporous Membrane with Backflushing to Minimize Fouling. Membranes 2023, 13, 707.
Lee, J.; Hong, J.; Lee, J.; Lee, C.; Kim, T.; Jeong, Y.; Kim, K.; Jung, I. Precise Filtration of Chronic Myeloid Leukemia Cells by an Ultrathin Microporous Membrane with Backflushing to Minimize Fouling. Membranes2023, 13, 707.
Lee, J.; Hong, J.; Lee, J.; Lee, C.; Kim, T.; Jeong, Y.; Kim, K.; Jung, I. Precise Filtration of Chronic Myeloid Leukemia Cells by an Ultrathin Microporous Membrane with Backflushing to Minimize Fouling. Membranes 2023, 13, 707.
Abstract
A cell filtration platform that affords accurate size separation and minimizes fouling was developed. The platform features an ultra-thin porous membrane (UPM) filter, a pumping head for backflushing (PHB), and cell size measurement (CSM) software. The UPM chip is an ultrathin free-standing membrane with a large window area of 0.68 mm2, a pore diameter of 5 to 9 μm, and a thickness of less than 0.9 μm. The PHB prevents filter fouling. The CSM software analyzes the size distributions of the supernatants and subnatants of isolated cells and presents the data visually. The D99 particle size of cells of the chronic myeloid leukemia (CML) line K562 decreased from 22.2 to 17.5 μm after passage through a 5-μm filter. K562 cells could be separated by careful selection of the pore size; the recovery rate attained 91.3%. The method was compared to conventional blocking models by evaluating the mean square errors (MSEs) between the measured and calculated filtering volumes. The filtering rate was fitted by a linear regression model with a significance that exceeded 0.99 based on the R2 value. The platform can be used to separate various soft biomaterials and affords excellent stability during filtration.
Keywords
ultra-thin porous membrane; pumping head for backflush; chronic myeloid leukemia cell; fouling; blocking filtration model
Subject
Engineering, Bioengineering
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
