PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
High-Quality Genome Assembly, Genetic Transformation and Green Fluorescent Protein Labeling of Lasiodiplodia theobromae Strain LTTK16-3, a Fungal Pathogen of Chinese Hickory Canker
Version 1
: Received: 4 July 2023 / Approved: 5 July 2023 / Online: 5 July 2023 (04:24:32 CEST)
How to cite:
Yan, C.; Liang, D.; Zhang, S.; Ma, T.; Zhang, C. High-Quality Genome Assembly, Genetic Transformation and Green Fluorescent Protein Labeling of Lasiodiplodia theobromae Strain LTTK16-3, a Fungal Pathogen of Chinese Hickory Canker. Preprints2023, 2023070260. https://doi.org/10.20944/preprints202307.0260.v1
Yan, C.; Liang, D.; Zhang, S.; Ma, T.; Zhang, C. High-Quality Genome Assembly, Genetic Transformation and Green Fluorescent Protein Labeling of Lasiodiplodia theobromae Strain LTTK16-3, a Fungal Pathogen of Chinese Hickory Canker. Preprints 2023, 2023070260. https://doi.org/10.20944/preprints202307.0260.v1
Yan, C.; Liang, D.; Zhang, S.; Ma, T.; Zhang, C. High-Quality Genome Assembly, Genetic Transformation and Green Fluorescent Protein Labeling of Lasiodiplodia theobromae Strain LTTK16-3, a Fungal Pathogen of Chinese Hickory Canker. Preprints2023, 2023070260. https://doi.org/10.20944/preprints202307.0260.v1
APA Style
Yan, C., Liang, D., Zhang, S., Ma, T., & Zhang, C. (2023). High-Quality Genome Assembly, Genetic Transformation and Green Fluorescent Protein Labeling of <em>Lasiodiplodia theobromae </em>Strain LTTK16-3, a Fungal Pathogen of Chinese Hickory Canker. Preprints. https://doi.org/10.20944/preprints202307.0260.v1
Chicago/Turabian Style
Yan, C., Tianling Ma and Chuanqing Zhang. 2023 "High-Quality Genome Assembly, Genetic Transformation and Green Fluorescent Protein Labeling of <em>Lasiodiplodia theobromae </em>Strain LTTK16-3, a Fungal Pathogen of Chinese Hickory Canker" Preprints. https://doi.org/10.20944/preprints202307.0260.v1
Abstract
Lasiodiplodia theobromae, as one of the causing agents associated with Chinese hickory trunk cankers, has caused huge economic losses to the Chinese hickory industry due to its extremely strong pathogenicity. Though the biological characteristics of this pathogen and the occurrence pattern of this disease have been reported, few studies have focused on investigating the mechanisms responsible for L. theobromae survival strategies and pathogenicity. The high-quality genome data and the efficient transformation system are the basis for researching above mechanism events. In this study we sequenced and assembled L. theobromae strain LTTK16-3, and established the first protoplasmic preparation method and polyethylene glycol (PEG) -mediated genetic transformation system for L. theobromae. These genetic information and transformation methods established the foundation for the future mechanisms study of L. theobromae and set up the possibility of targeted molecular improvements for Chinese hickory canker control.
Keywords
Genome; transformation; Lasiodiplodia theobromae; Tree trunk canker; Chinese hickory
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
