Version 1
: Received: 1 July 2023 / Approved: 3 July 2023 / Online: 3 July 2023 (13:23:21 CEST)
Version 2
: Received: 25 July 2023 / Approved: 25 July 2023 / Online: 25 July 2023 (10:42:03 CEST)
Version 3
: Received: 7 August 2023 / Approved: 8 August 2023 / Online: 8 August 2023 (11:09:48 CEST)
Version 4
: Received: 28 August 2023 / Approved: 28 August 2023 / Online: 28 August 2023 (08:27:47 CEST)
How to cite:
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Ul Haq, A.; Wang, J. Chemicals in Essential Oils of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen and Their Antioxidant Activities Potential. Preprints2023, 2023070092. https://doi.org/10.20944/preprints202307.0092.v4
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Ul Haq, A.; Wang, J. Chemicals in Essential Oils of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen and Their Antioxidant Activities Potential. Preprints 2023, 2023070092. https://doi.org/10.20944/preprints202307.0092.v4
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Ul Haq, A.; Wang, J. Chemicals in Essential Oils of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen and Their Antioxidant Activities Potential. Preprints2023, 2023070092. https://doi.org/10.20944/preprints202307.0092.v4
APA Style
Pan, Z., Xie, C., Yan, X.T., Su, Y.M., Ul Haq, A., & Wang, J. (2023). Chemicals in Essential Oils of <em><i>Phlomoides rotata</i></em> (Benth. ex Hook. f.) Mathiesen and Their Antioxidant Activities Potential. Preprints. https://doi.org/10.20944/preprints202307.0092.v4
Chicago/Turabian Style
Pan, Z., Anwar Ul Haq and Jian Wang. 2023 "Chemicals in Essential Oils of <em><i>Phlomoides rotata</i></em> (Benth. ex Hook. f.) Mathiesen and Their Antioxidant Activities Potential" Preprints. https://doi.org/10.20944/preprints202307.0092.v4
Abstract
Due to the low content, few studies are focused on the essential oils (EOs) of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen (syn. Lamiophlomis rotata (Benth.) Kudô. This plant has been used to treat rheumatic arthritis and grasserie in China. However, such EOs may have important pharmacological activities such as anti-cancer. To identify the chemical markers (CM) in the EOs and evaluate their antioxidant activities (AAs), we firstly conduct a thoroughly investigation on their chemicals and AAs, to the best of our knowledge. Light yellow EOs with fresh and elegant smell are obtained by hydro-distillation with average yield 0.11% (volume mL/weight g). The crystals are separated from the EOs through cryoprecipitation, respectively. A total of 81 components are qualified and quantified in the EOs, crystals and EOs removed crystals, in which 44 ones are first reported. As for content, the main compounds are long-chain fatty acids (FAs) and their esters. The most abundant one n-hexadecanoic acid (palmitic acid, PA) accounting for 43.15-54.8%, 58.49-64.57% and 15.9-41.1%, in the EOs, crystals and EOs removed crystals, respectively. Seven compounds including PA, tetradecanoic acid, linoleic acid, oleic acid, methyl hexadecanoate, hexahydrofarnesyl acetone and phytol can be chosen a the CMs in these EOs. The AAs are evaluated through in vitro assays. PA presents pro-oxidant activities in a concentration dependence manner. Usually, the EOs removed crystals demonstrate stronger AAs, and the crystals demonstrate weaker AAs compared with that of the corresponding EOs, which is related to the different content of PA in these samples. This study can give some hints for the utilization of such EOs which are abundant in FAs such as PA.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
28 August 2023
Commenter:
Jian Wang
Commenter's Conflict of Interests:
Author
Comment:
After fully considering the reviewers’ comment, the article title has been changed to “Chemicals in Essential Oils of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen and Their Antioxidant Activities Potential”. Some change have been made. For example, the last a few paragraphs in 2.2. have been combined into one paragraph and some “prominent” has been rephrased. In addition, besides the former 55 compounds, another 26 compounds have been identified and quantified, which can be seen in Table 1. As a result, 44 compounds are first reported from the essential oils of Phlomoides rotata, or even the first time from this plant, to the best of our knowledge.
Commenter: Jian Wang
Commenter's Conflict of Interests: Author
Some change have been made. For example, the last a few paragraphs in 2.2. have been combined into one paragraph and some “prominent” has been rephrased. In addition, besides the former 55 compounds, another 26 compounds have been identified and quantified, which can be seen in Table 1. As a result, 44 compounds are first reported from the essential oils of Phlomoides rotata, or even the first time from this plant, to the best of our knowledge.