Version 1
: Received: 1 July 2023 / Approved: 3 July 2023 / Online: 3 July 2023 (13:23:21 CEST)
Version 2
: Received: 25 July 2023 / Approved: 25 July 2023 / Online: 25 July 2023 (10:42:03 CEST)
Version 3
: Received: 7 August 2023 / Approved: 8 August 2023 / Online: 8 August 2023 (11:09:48 CEST)
Version 4
: Received: 28 August 2023 / Approved: 28 August 2023 / Online: 28 August 2023 (08:27:47 CEST)
How to cite:
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Ul Haq, A.; Wang, J. Chemicals in Essential Oils of Lamiophlomis rotata (Benth.) Kudo and Their Antioxidant Activities. Preprints2023, 2023070092. https://doi.org/10.20944/preprints202307.0092.v3
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Ul Haq, A.; Wang, J. Chemicals in Essential Oils of Lamiophlomis rotata (Benth.) Kudo and Their Antioxidant Activities. Preprints 2023, 2023070092. https://doi.org/10.20944/preprints202307.0092.v3
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Ul Haq, A.; Wang, J. Chemicals in Essential Oils of Lamiophlomis rotata (Benth.) Kudo and Their Antioxidant Activities. Preprints2023, 2023070092. https://doi.org/10.20944/preprints202307.0092.v3
APA Style
Pan, Z., Xie, C., Yan, X.T., Su, Y.M., Ul Haq, A., & Wang, J. (2023). Chemicals in Essential Oils of <em>Lamiophlomis rotata</em> (Benth.) Kudo and Their Antioxidant Activities. Preprints. https://doi.org/10.20944/preprints202307.0092.v3
Chicago/Turabian Style
Pan, Z., Anwar Ul Haq and Jian Wang. 2023 "Chemicals in Essential Oils of <em>Lamiophlomis rotata</em> (Benth.) Kudo and Their Antioxidant Activities" Preprints. https://doi.org/10.20944/preprints202307.0092.v3
Abstract
Due to the low content, few studies are focused on the essential oils (EOs) of Lamiophlomis rotata (Benth.) Kudo (L. rotata), which has been used to treat rheumatic arthritis and grasserie in China. However, such EOs may have important pharmacological activities such as anti-cancer. To identify the chemical markers (CM) in the EOs and evaluate their antioxidant activities (AAs), we firstly conduct a thoroughly investigation on their chemicals and AAs, to the best of our knowledge. Light yellow EOs with fresh and elegant smell are obtained by hydro-distillation with average yield 0.11% (volume mL/weight g). The crystals are separated from the EOs through cryoprecipitation, respectively. A total of 55 components are qualified and quantified in the EOs, crystals and EOs removed crystals, in which 21 ones are first reported. As for content, the main compounds are long-chain fatty acids (FAs) and their esters. The most abundant one n-hexadecanoic acid (palmitic acid, PA) accounting for 47.1-60.8%, 61.3-69.2% and 17.8-44.8%, in the EOs, crystals and EOs removed crystals, respectively. Seven compounds including PA, tetradecanoic acid, linoleic acid, oleic acid, methyl hexadecanoate, hexahydrofarnesyl acetone and phytol can be chosen a the CMs in these EOs. The AAs are evaluated through in vitro assays. Three kinds of EOs and EOs removed crystals present some AAs, but not so strong compared with that of ascorbic acid. PA presents pro-oxidant activities in a concentration dependence manner. Usually, the EOs removed crystals demonstrate stronger AAs, and the crystals demonstrate weaker AAs compared with that of the corresponding EOs, which is related to the different content of PA in these samples. This study can give some hints for the utilization of such EOs which are abundant in FAs such as PA.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
8 August 2023
Commenter:
Jian Wang
Commenter's Conflict of Interests:
Author
Comment:
For example, the figures showing the antioxidant activities results are deleted; the reasons for studying the essential oils (EOs), crystals and EOs removed crystals are clarified in the last paragraph of introduction section. In addition, Supplemental Table 1 including the data of chemicals identified in EOs and lipophilic part of Lamiophlomis rotata in previous studies has been added in the introduction; The data gotten from GC-FID and GC-MS using DB-5 in Table 1 have been deleted; The graphical abstract has been improved.
Commenter: Jian Wang
Commenter's Conflict of Interests: Author