Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Chemicals in Essential Oils of Lamiophlomis rotata (Benth.) Kudo and Their Antioxidant Activities

Version 1 : Received: 1 July 2023 / Approved: 3 July 2023 / Online: 3 July 2023 (13:23:21 CEST)
Version 2 : Received: 25 July 2023 / Approved: 25 July 2023 / Online: 25 July 2023 (10:42:03 CEST)
Version 3 : Received: 7 August 2023 / Approved: 8 August 2023 / Online: 8 August 2023 (11:09:48 CEST)
Version 4 : Received: 28 August 2023 / Approved: 28 August 2023 / Online: 28 August 2023 (08:27:47 CEST)

How to cite: Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Ul Haq, A.; Wang, J. Chemicals in Essential Oils of Lamiophlomis rotata (Benth.) Kudo and Their Antioxidant Activities. Preprints 2023, 2023070092. https://doi.org/10.20944/preprints202307.0092.v1 Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Ul Haq, A.; Wang, J. Chemicals in Essential Oils of Lamiophlomis rotata (Benth.) Kudo and Their Antioxidant Activities. Preprints 2023, 2023070092. https://doi.org/10.20944/preprints202307.0092.v1

Abstract

Due to the low content, very few studies were focused on the essential oils (EOs) of Lamiophlomis rotata (Benth.) Kudo (L. rotata), which has been used to treat rheumatic arthritis and grasserie in China. However, such EOs may have important pharmacological activities such as anti-cancer. To explore the potential of L. rotata, we firstly conducted a thoroughly investigation on the chemicals in its EOs and their antioxidant activities, to the best of our knowledge. Light yellow EOs with fresh and elegant smell were obtained by hydro-distillation with percentage yields of 0.08-0.13% (volume mL/weight g). The crystals were separated from these EOs further. Therefore, the EOs, crystals, and EOs removed crystals were studied in-depth. A total of 56 components were qualified and quantified, in which 22 ones were first reported in such EOs. The main compounds were long-chain fatty acids and their esters. Seven compounds included n-hexadecanoic acid (47.1-60.8%), tetradecanoic acid (2.8-3.9%), linoleic acid (2.7-7.7%), oleic acid (0-3.7%), hexadecanoic acid, methyl ester (1.5-3.9%), 2-pentadecanone, 6,10,14-trimethyl- (2.0-3.0%), and phytol (2.0-5.8%) were prominent. The crystals were mainly composed of n-hexadecanoic acid with content 61.3-69.2%. Their antioxidant activities were evaluated through three common methods such as DPPH (1,1-Diphenyl-2-picrylhydrazy1 radical), ABTS ((2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), and FRAP (ferric reducing/antioxidant power). Three kinds of EOs and EOs removed crystals present some antioxidant activities, but insignificant compared with that of ascorbic acid. The crystals showed nearly non antioxidant activities and even oxidant activities, and the same situation for palmitic acid. As a result, the EOs removed crystals usually showed some stronger antioxidant activities than that of corresponding EOs. This study will give some hints for the full usage of such EOs with lower extracted rate and mainly composed of compounds with high boiling point such as n-hexadecanoic acid, 6,10,14-trimethyl-2-pentadecanone, etc..

Keywords

Lamiophlomis rotata (Benth.) Kudo; essential oils; n-hexadecanoic acid (palmitic acid); 6,10,14-trimethyl-2-pentadecanone (hexahydrofarnesyl acetone); chemical markers; antioxidant activities

Subject

Medicine and Pharmacology, Pharmacy

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