Version 1
: Received: 27 June 2023 / Approved: 29 June 2023 / Online: 30 June 2023 (03:02:32 CEST)
How to cite:
Phan, M.; Pham-Thi, N.; Nguyen, H.; Dinh, V. Growth Characteristics and Efficacy of Umbilical Cord Lining Mesenchymal Stem Cells in Burn Wound Treatment. Preprints2023, 2023062119. https://doi.org/10.20944/preprints202306.2119.v1
Phan, M.; Pham-Thi, N.; Nguyen, H.; Dinh, V. Growth Characteristics and Efficacy of Umbilical Cord Lining Mesenchymal Stem Cells in Burn Wound Treatment. Preprints 2023, 2023062119. https://doi.org/10.20944/preprints202306.2119.v1
Phan, M.; Pham-Thi, N.; Nguyen, H.; Dinh, V. Growth Characteristics and Efficacy of Umbilical Cord Lining Mesenchymal Stem Cells in Burn Wound Treatment. Preprints2023, 2023062119. https://doi.org/10.20944/preprints202306.2119.v1
APA Style
Phan, M., Pham-Thi, N., Nguyen, H., & Dinh, V. (2023). Growth Characteristics and Efficacy of Umbilical Cord Lining Mesenchymal Stem Cells in Burn Wound Treatment. Preprints. https://doi.org/10.20944/preprints202306.2119.v1
Chicago/Turabian Style
Phan, M., Hong-Ha Nguyen and Van-Han Dinh. 2023 "Growth Characteristics and Efficacy of Umbilical Cord Lining Mesenchymal Stem Cells in Burn Wound Treatment" Preprints. https://doi.org/10.20944/preprints202306.2119.v1
Abstract
Burn injuries have profound implications for aesthetics, functionality, and patient survival. Umbilical cord lining mesenchymal stem cells (SCL-MSCs) have emerged as a potential therapeutic approach for burn wounds due to their easy isolation, favorable differentiation capabilities, and low immunogenicity. This study aimed to evaluate the growth characteristics of SCL-MSCs and their effectiveness in treating burn wounds. Mechanical isolation was employed to extract mesenchymal stem cells from the umbilical cord lining membrane. The isolated cells underwent characterization, including analysis of HLA-E, G, and DR expression, and assessment of immunogenicity against rabbit antigens using ELISA. A rabbit model was utilized to administer burn treatment. SCL-MSCs exhibited typical morphology of mesenchymal stem cells and demonstrated robust proliferation. These cells expressed CD90, HLA-E, and HLA-G markers while lacking HLA-DR expression. Intact cells and supercrushed cell fluid displayed minimal and unstable immunogenicity. SCL-MSCs exhibited remarkable differentiation potential toward fibroblast lineages, with differentiated fibroblasts showing elevated collagen production and efficient sheet formation. In the experimental rabbit model, burn wounds covered with SCL-MSC tegaderm material plates exhibited superior healing compared to controls. The stem cell material plate area demonstrated reduced inflammatory cell counts (P < 0.01) and increased fibroblast count, neovascularization, and mitotic index (P < 0.01). These findings highlight the potential of SCL-MSCs as a valuable resource for burn wound research and treatment.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
