Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Metabolome Mining of Curcuma longa L. Using HPLC-MS/MS and Molecular Networking

Rabin Budhathoki
ORCID logo ,
Arjun Prasad Timilsina
ORCID logo ,
Bishnu P. Regmi
,
Khaga Raj Sharma
ORCID logo ,
Niraj Aryal
* ORCID logo ,
Niranjan Parajuli
* ORCID logo
Version 1 : Received: 27 June 2023 / Approved: 27 June 2023 / Online: 27 June 2023 (04:47:44 CEST)

A peer-reviewed article of this Preprint also exists.

Budhathoki, R.; Timilsina, A.P.; Regmi, B.P.; Sharma, K.R.; Aryal, N.; Parajuli, N. Metabolome Mining of Curcuma longa L. Using HPLC-MS/MS and Molecular Networking. Metabolites 2023, 13, 898. Budhathoki, R.; Timilsina, A.P.; Regmi, B.P.; Sharma, K.R.; Aryal, N.; Parajuli, N. Metabolome Mining of Curcuma longa L. Using HPLC-MS/MS and Molecular Networking. Metabolites 2023, 13, 898.

Abstract

Turmeric, Curcuma longa L., is a type of medicinal plant characterized by its perennial nature and rhizomatous growth. It is a member of the Zingiberaceae family and is distributed across the world’s tropical and subtropical climates, especially in South Asia. Its rhizomes are highly valued for food supplements, spices, flavoring agents, and yellow dye in South Asia since ancient times. It exhibits a diverse array of therapeutic qualities that encompass its ability to combat diabetes, reduce inflammation, act as an antioxidant, exhibit anticancer properties, and promote anti-aging effects. In this study, organic extracts of C. longa rhizomes were subjected to HPLC separation followed by mass spectrometry analysis. The Global Natural Product Social Molecular Networking (GNPS) approach was utilized for the first time in this ethnobotanically important species to conduct an in-depth analysis of its metabolomes based on their fragments. A total of 30 metabolites including 16 diarylheptanoids, 1 diarylpentanoid, 3 bisabolocurcumin ethers, 4 sesquiterpenoids, 4 cinnamic acid derivatives, and 2 fatty acid derivatives were identified. Among 16 diarylheptanoids identified in this study, five of them are reported for the first time in this species.

Keywords

Untargeted metabolomics; HPLC-ESI-MS/MS; Curcuma longa; GNPS; Diarylheptanoids

Subject

Chemistry and Materials Science, Analytical Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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