PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Molecular Characterization of a Male-specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone
Version 1
: Received: 13 June 2023 / Approved: 14 June 2023 / Online: 14 June 2023 (04:52:38 CEST)
Version 2
: Received: 27 June 2023 / Approved: 28 June 2023 / Online: 28 June 2023 (10:11:39 CEST)
Jiang, Q.; Xu, D.; Wang, M.; Xie, X.; Zhu, D. Molecular Characterization of a Male-Specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone. Fishes2023, 8, 351.
Jiang, Q.; Xu, D.; Wang, M.; Xie, X.; Zhu, D. Molecular Characterization of a Male-Specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone. Fishes 2023, 8, 351.
Jiang, Q.; Xu, D.; Wang, M.; Xie, X.; Zhu, D. Molecular Characterization of a Male-Specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone. Fishes2023, 8, 351.
Jiang, Q.; Xu, D.; Wang, M.; Xie, X.; Zhu, D. Molecular Characterization of a Male-Specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone. Fishes 2023, 8, 351.
Abstract
The SRY-related HMG-box (Sox) family is a group of transcriptional regulators that play critical role in many important developmental processes in both vertebrates and invertebrates. In aquatic animals, the function of Sox genes on sexual development has been gained particular attentions. The present study reported the molecular characterization of a SoxE members (PtSoxE) in the swimming crab, Portunus trituberculatus, and tissue distribution analysis showed it was male-specific. Since the most highly expression of PtSoxE was found in the androgenic gland (AG), its relation to the insulin-like androgenic gland hormone (IAG) was further investigated. The PtSoxE siRNA caused significantly decrease in IAG expression in both AG and testis, whereas the PtSoxE expression could also be induced by treating with AG homogenate and rIAG. The result suggested a transcriptional interaction between PtSoxE and IAG. In addition, the PtSoxE expression showed a closely positively correlation with several reported spermatogenesis-related genes, suggesting its involvement in the testicular development of P. trituberculatus.
Keywords
Portunus trituberculatus; SoxE gene; insulin-like androgenic gland hormone; testicular development
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
