Age-Dependent Molecular and Metabolic Rewiring Leading to Wound-Induced Blackening in Cut Carrots

Katie Schulz; Gabriela Machaj; Paul Knox; Robert Hancock; Susan R Verrall; Risto Korpinen; Pekka Saranpaa; Anna Happonen; Barbara Karpinska; Christine Helen Foyer

doi:10.20944/preprints202306.0225.v2

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preprints.org > biology and life sciences > plant sciences > doi: 10.20944/preprints202306.0225.v2

Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Age-Dependent Molecular and Metabolic Rewiring Leading to Wound-Induced Blackening in Cut Carrots

Christine Helen Foyer

Version 1 : Received: 2 June 2023 / Approved: 5 June 2023 / Online: 5 June 2023 (02:43:49 CEST)
Version 2 : Received: 5 June 2023 / Approved: 6 June 2023 / Online: 6 June 2023 (03:57:18 CEST)

How to cite: Schulz, K.; Machaj, G.; Knox, P.; Hancock, R.; Verrall, S.R.; Korpinen, R.; Saranpaa, P.; Happonen, A.; Karpinska, B.; Foyer, C.H. Age-Dependent Molecular and Metabolic Rewiring Leading to Wound-Induced Blackening in Cut Carrots. Preprints 2023, 2023060225. https://doi.org/10.20944/preprints202306.0225.v2 Schulz, K.; Machaj, G.; Knox, P.; Hancock, R.; Verrall, S.R.; Korpinen, R.; Saranpaa, P.; Happonen, A.; Karpinska, B.; Foyer, C.H. Age-Dependent Molecular and Metabolic Rewiring Leading to Wound-Induced Blackening in Cut Carrots. Preprints 2023, 2023060225. https://doi.org/10.20944/preprints202306.0225.v2

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MDPI and ACS Style

Schulz, K.; Machaj, G.; Knox, P.; Hancock, R.; Verrall, S.R.; Korpinen, R.; Saranpaa, P.; Happonen, A.; Karpinska, B.; Foyer, C.H. Age-Dependent Molecular and Metabolic Rewiring Leading to Wound-Induced Blackening in Cut Carrots. Preprints 2023, 2023060225. https://doi.org/10.20944/preprints202306.0225.v2

APA Style

Schulz, K., Machaj, G., Knox, P., Hancock, R., Verrall, S.R., Korpinen, R., Saranpaa, P., Happonen, A., Karpinska, B., & Foyer, C.H. (2023). Age-Dependent Molecular and Metabolic Rewiring Leading to Wound-Induced Blackening in Cut Carrots. Preprints. https://doi.org/10.20944/preprints202306.0225.v2

Chicago/Turabian Style

Schulz, K., Barbara Karpinska and Christine Helen Foyer. 2023 "Age-Dependent Molecular and Metabolic Rewiring Leading to Wound-Induced Blackening in Cut Carrots" Preprints. https://doi.org/10.20944/preprints202306.0225.v2

Abstract

The blackening of cut carrots decreases their shelf life and causes severe economic losses but the molecular and metabolic mechanisms that underpin this phenomenon remain poorly characterized. Studies were therefore undertaken to determine the molecular and metabolic causes of the blackening. The susceptibility of blackening was dependent on the period of time that the crop was stored underground prior to harvest. The structure of the cell walls in the black regions was substantially changed compared to the orange regions. The black regions of carrot batons had decreased immunodetection of xyloglucan, HG-pectin, RG-I pectin, galactan and arabinan but had higher levels of lignin and phenolic compounds compared to the orange regions. Transcript profiling analysis revealed that phytohormone signalling processes were activated in the black regions. Transcripts associated with auxin signalling and ethylene-responsive transcription factors were increased in the black regions. In contrast, the levels of transcripts encoding proteins associated with secondary metabolism were decreased in the black regions. These findings implicate ethylene and auxin-related processes in the control of the primary to secondary metabolism shift that results in lignification and cell wall disruption that underpin the blackening process.

Keywords

Auxin; cell wall composition; chlorogenic acid; lignin; pectin; post harvest wounding; tap roots

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Received: 6 June 2023
Commenter: Christine Foyer
Commenter's Conflict of Interests: Author

Comment: Last name of first author spelt incorrectly

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