Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Rapid Prototyping of 3D Printed AgNPs and Nano-TiO2 Embedded Hydrogels as Novel Devices with Multi-Responsive Antimicrobial Capability in Wound Healing

Version 1 : Received: 26 May 2023 / Approved: 29 May 2023 / Online: 29 May 2023 (04:41:53 CEST)

A peer-reviewed article of this Preprint also exists.

Remaggi, G.; Bergamonti, L.; Graiff, C.; Ossiprandi, M.C.; Elviri, L. Rapid Prototyping of 3D-Printed AgNPs- and Nano-TiO2-Embedded Hydrogels as Novel Devices with Multiresponsive Antimicrobial Capability in Wound Healing. Antibiotics 2023, 12, 1104. Remaggi, G.; Bergamonti, L.; Graiff, C.; Ossiprandi, M.C.; Elviri, L. Rapid Prototyping of 3D-Printed AgNPs- and Nano-TiO2-Embedded Hydrogels as Novel Devices with Multiresponsive Antimicrobial Capability in Wound Healing. Antibiotics 2023, 12, 1104.

Abstract

Two antimicrobial agents such as silver nanoparticles (AgNPs) and titanium dioxide (TiO2) have been formulated with natural polysaccharides (chitosan or alginate) to develop innovative inks for the rapid, customizable, and extremely accurate manufacturing of 3D printed scaffolds useful as dressings in the treatment of infected skin wounds. Suitable chemical-physical properties for the applicability of these innovative devices were demonstrated through the evaluation of water content (88-93%), mechanical strength (Young’s modulus 0.23-0.6 MPa), elasticity, and morphology. The antimicrobial tests performed against Staphylococcus Aureus and Pseudomonas Aeruginosa demonstrated the antimicrobial activities against Gram+ and Gram– bacteria of AgNPs and TiO2 agents embedded in the CH or ALG macroporous 3D hydrogels (AgNPs MIC starting from 5 µg/ml). The biocompatibility of chitosan was widely demonstrated by cell viability tests and was higher than that observed for alginate. Constructs containing AgNPs at 10 µg/ml concentration level did not significantly alter cell viability as well as the presence of titanium dioxide; cytotoxicity towards human fibroblasts was observed starting with AgNPs concentration of 100 µg/ml. In conclusions, the 3D printed dressings here developed own the features to be cheap, highly defined, easy to be manufactured and further applied in personalized antimicrobial medicine applications.

Keywords

3D printing; silver nanoparticles; titanium dioxide; hydrogels; antimicrobial activity

Subject

Biology and Life Sciences, Life Sciences

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