Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isoespintanol Antifungal Activity Involves Mitochondrial Dysfunction, Inhibition of Biofilm Formation, and Damage to Cell Wall Integrity in Candida tropicalis

Orfa Inés Contreras Martínez
* ORCID logo ,
Alberto Angulo Ortíz
,
Gilmar Santafé Patiño
ORCID logo ,
Ana Peñata-Taborda
ORCID logo ,
Ricardo Berrio Soto
Version 1 : Received: 18 May 2023 / Approved: 19 May 2023 / Online: 19 May 2023 (10:04:23 CEST)

A peer-reviewed article of this Preprint also exists.

Contreras Martínez, O.I.; Angulo Ortíz, A.; Santafé Patiño, G.; Peñata-Taborda, A.; Berrio Soto, R. Isoespintanol Antifungal Activity Involves Mitochondrial Dysfunction, Inhibition of Biofilm Formation, and Damage to Cell Wall Integrity in Candida tropicalis. Int. J. Mol. Sci. 2023, 24, 10187. Contreras Martínez, O.I.; Angulo Ortíz, A.; Santafé Patiño, G.; Peñata-Taborda, A.; Berrio Soto, R. Isoespintanol Antifungal Activity Involves Mitochondrial Dysfunction, Inhibition of Biofilm Formation, and Damage to Cell Wall Integrity in Candida tropicalis. Int. J. Mol. Sci. 2023, 24, 10187.

Abstract

The growing increase in infections caused by C. tropicalis, associated with its drug resistance and consequent high mortality, especially in immunosuppressed people, today generates a serious global public health problem. In the search for new potential drug candidates that can be used as treatments or adjuvants in the control of infections by these pathogenic yeasts, the objective of this research was to evaluate the action of isoespintanol (ISO) against the formation of biofilms fungal, the mitochondrial membrane potential (∆Ψm) and its effect on the integrity of the cell wall. We report the ability of ISO to inhibit the formation of biofilms by up to 89.35%, in all cases higher than the values expressed by amphotericin B (AFB). Flow cytometric experiments using rhodamine 123 (Rh123) showed the ability of ISO to cause mitochondrial dysfunction in these cells. Likewise, experiments using calcofluor white (CFW) and analyzed by flow cytometry, showed the ability of ISO to affect the integrity of the cell wall by stimulating chitin synthesis; these changes in the integrity of the wall were also observed through transmission electron microscopy (TEM). These mechanisms are involved in the antifungal action of this monoterpene.

Keywords

antifungal mechanism; isoespintanol; Candida tropicalis; antibiofilm activity

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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