Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Multi-Omics Analysis of Vicia cracca Responses to Chronic Radiation Exposure in the Chernobyl Exclusion Zone

Viktoria Voronezhskaya
,
Polina Volkova
* ORCID logo ,
Sofia Bitarishvili
,
Ekaterina Shesterikova
,
Mikhail Podlutskii
,
Gilles Clement
ORCID logo ,
Christian Meyer
,
Gustavo T. Duarte
ORCID logo ,
Maksim Kudin
,
Dmitrii Garbaruk
,
Larisa Turchin
,
Elizaveta Kazakova
* ORCID logo
Version 1 : Received: 11 May 2023 / Approved: 12 May 2023 / Online: 12 May 2023 (10:39:57 CEST)

A peer-reviewed article of this Preprint also exists.

Voronezhskaya, V.; Volkova, P.; Bitarishvili, S.; Shesterikova, E.; Podlutskii, M.; Clement, G.; Meyer, C.; Duarte, G.T.; Kudin, M.; Garbaruk, D.; Turchin, L.; Kazakova, E. Multi-Omics Analysis of Vicia cracca Responses to Chronic Radiation Exposure in the Chernobyl Exclusion Zone. Plants 2023, 12, 2318. Voronezhskaya, V.; Volkova, P.; Bitarishvili, S.; Shesterikova, E.; Podlutskii, M.; Clement, G.; Meyer, C.; Duarte, G.T.; Kudin, M.; Garbaruk, D.; Turchin, L.; Kazakova, E. Multi-Omics Analysis of Vicia cracca Responses to Chronic Radiation Exposure in the Chernobyl Exclusion Zone. Plants 2023, 12, 2318.

Abstract

Our understanding of the long-term consequences of chronic ionising radiation for living organisms remains scarce. Modern molecular biology techniques are helpful tools for researching pollutant effects on biota. To reveal the molecular phenotype of plants growing under chronic radiation exposure, we sampled Vicia cracca L. plants in the Chernobyl Exclusion Zone and in areas with normal radiation backgrounds. We performed a detailed analysis of soil and gene expression patterns, and made coordinated multi-omics analyses of plant samples, including transcriptomics, proteomics, and metabolomics. Chronic exposure to ionising radiation induced complex and multidirectional changes, including significant alterations in the metabolism and gene expression patterns of irradiated plants. We revealed profound changes in carbon metabolism, nitrogen reallocation, and photosynthesis. These plants were characterised by increased DNA damage, redox imbalance, and stress response reactions. The upregulation of histones, chaperones, peroxidases, and secondary metabolism were noted.

Keywords

Fabaceae; transcriptomics; proteomics; metabolomics; low doses; abiotic stress

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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