Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Phytochemical Characterization and Biological Activities of Stenomesson miniatum Bulb Extract, a Medicinal Plant of the Andes

Mariacaterina Lianza
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Ferruccio Poli
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Carmela Fimognari
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Jean-Marc Nuzillard
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Jean-Hugues Renault
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Francesca Bonvicini
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Giulia Greco
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Eleonora Turrini
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Version 1 : Received: 11 May 2023 / Approved: 12 May 2023 / Online: 12 May 2023 (10:12:45 CEST)
Version 2 : Received: 12 December 2023 / Approved: 12 December 2023 / Online: 14 December 2023 (12:10:15 CET)

A peer-reviewed article of this Preprint also exists.

Lianza, M.; Fimognari, C.; Nuzillard, J.-M.; Poli, F.; Renault, J.-H.; Bonvicini, F.; Greco, G.; Turrini, E. Phytochemical Characterization and Biological Activities of Stenomesson Miniatum Bulb Extract, a Medicinal Plant of the Andes. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 2023, 1–14, doi:10.1080/11263504.2023.2293037. Lianza, M.; Fimognari, C.; Nuzillard, J.-M.; Poli, F.; Renault, J.-H.; Bonvicini, F.; Greco, G.; Turrini, E. Phytochemical Characterization and Biological Activities of Stenomesson Miniatum Bulb Extract, a Medicinal Plant of the Andes. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 2023, 1–14, doi:10.1080/11263504.2023.2293037.

Abstract

The fresh bulbs of Stenomesson miniatum, a plant belonging to Amaryllidaceae family with a poorly investigated phytochemical profile, were traditionally employed by Andean healers to treat tumors and abscesses. The aims of this study were to characterize the alkaloid extract from the bulbs of S. miniatum and test its cytotoxic and antibacterial potential. The alkaloid extract was characterized by dereplication using various techniques (CPC, NMR, UPLC-HRMS) and referring to in-home or online databases for spectroscopic data matching. Cytotoxic activities were evaluated on A431 human epidermoid carcinoma cells through a metabolic assay, and on Jurkat human acute T-leukemia cells through a cell-impermeant fluorescent nuclear probe. Antibacterial assays were carried out against Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes by using a standardized microdilution broth method 96-well plate. Eleven known Amaryllidaceae alkaloids were identified together with another compound determined as being an extraction artefact. The alkaloid extract showed good cytotoxic activity against both the tumor cell lines, reaching an IC50 of 3.3 µg/mL against A431 cells and of 10.9 µg/mL against Jurkat cells. The analysis of the fractions allowed the activity to be attributed to the presence of pretazettine and haemathamine. Conversely, no antibacterial activities were recorded for any of the samples.

Keywords

Stenomesson miniatum; Amaryllidaceae alkaloids; Urceolina peruviana; 13C-NMR-based dereplication; A431 human epidermoid carcinoma; skin cancer; Jurkat cells; artefact; Andean traditional medicine

Subject

Chemistry and Materials Science, Medicinal Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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