Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Chemical Profiling of Diffusible and Volatile Secondary Metab-Olites Produced by Beauveria bassiana Using GC-MS Analysis: In Vitro Antimicrobial Activity

Version 1 : Received: 6 April 2023 / Approved: 10 April 2023 / Online: 10 April 2023 (03:06:01 CEST)

How to cite: Camele, I.; Sadeek, S.A.; Racioppi, R.; Elshafie, H.S. Chemical Profiling of Diffusible and Volatile Secondary Metab-Olites Produced by Beauveria bassiana Using GC-MS Analysis: In Vitro Antimicrobial Activity. Preprints 2023, 2023040134. https://doi.org/10.20944/preprints202304.0134.v1 Camele, I.; Sadeek, S.A.; Racioppi, R.; Elshafie, H.S. Chemical Profiling of Diffusible and Volatile Secondary Metab-Olites Produced by Beauveria bassiana Using GC-MS Analysis: In Vitro Antimicrobial Activity. Preprints 2023, 2023040134. https://doi.org/10.20944/preprints202304.0134.v1

Abstract

The genus Beauveria include important entomopathogenic and endophytic fungi, among them, B. bassiana is the most studied species. However, there are few knowledge regarding their antimicrobial activity. The current research has been conducted to evaluate in vitro antibacterial efficacy of five isolates of B. bassiana against Bacillus cereus, B. megaterium, B. mojavensis, Clavibacter michiganensis (gram positive bacteria, G+ve), Xanthomonas campestris, X. vesicatoria, Escherichia coli, Pseudomonas aeruginosa and P. fluorescence (gram negative bacteria, G-ve). In addition, chemical composition of the principal diffusible metabolites and volatile organic compounds (VOCs) of the most efficient studied isolate of B. bassiana has been carried out using GC-MS analysis. The obtained results showed that, the isolate UniB2439-3 has promising antibacterial effect against most of studied target bacteria. GC-MS analysis of diffusible metabolites detected the presence of hexanedioic acid, bis(2-ethylhexyl) ester as the main compound in the cell-free culture filtrate. Furthermore, GC-MS analysis of VOCs revealed the presence of ethanol; butanal,2-methyl; 2,4-dimethyl-1-heptene; octane, 4-methyl and β-elemene as the main dominant bioactive compounds. The outgoing results explicated that, the isolates of B. bassiana have promising antibacterial activity which could be correlated to their diffusible and VOCs metabolites. Therefore, the selected isolate can be potentially used as a biocontrol agent against several bacteria especially G+ve ones. Taking in consideration that the antibiotics are forbidden in agriculture in many countries worldwide, search for possible natural alternatives as efficient antimicrobial agents are highly interesting.

Keywords

biocontrol; natural products; phytopathogens; microbial metabolites; entomopathogens; endophytic fungi

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.