Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047

Svetlana Vasilieva
,
Karina Shibzukhova
ORCID logo ,
Alexei Solovchenko
* ORCID logo ,
Olga Chivkunova
,
Kristina Antipova
,
Alexey Morozov
,
Elena Lobakova
Version 1 : Received: 14 March 2023 / Approved: 15 March 2023 / Online: 15 March 2023 (10:45:27 CET)

A peer-reviewed article of this Preprint also exists.

Vasilieva, S.; Shibzukhova, K.; Solovchenko, A.; Chivkunova, O.; Antipova, C.; Morozov, A.; Lobakova, E. Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047. J. Mar. Sci. Eng. 2023, 11, 865. Vasilieva, S.; Shibzukhova, K.; Solovchenko, A.; Chivkunova, O.; Antipova, C.; Morozov, A.; Lobakova, E. Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047. J. Mar. Sci. Eng. 2023, 11, 865.

Abstract

Green microalgae including those from the genus Lobosphaera are exploited in biotechnology to obtain valuable fatty acids e.g., arachidonic acid (C20:4, ARA) for the production of infant formulae, food and feed additives. In nature, microalgae frequently exist in naturally immobilized state (as biofilms) with limited cell division rate and increased stress resilience. In biotechnology, immobilization of microalgae on artificial cell carriers simplifies biomass harvesting, increases culture robustness and productivity. The choice of suitable cell carrier is central to biotechnology of immobilized culture. Cell carriers based on the natural amine-containing polymer chitosan and synthetic polyethylenimine (PEI) are promising candidates for immobilization of phototrophic microorganisms. This is the first report on the effects of immobilization on PEI and chitosan on the accumulation and composition of polyunsaturated fatty acids, including ARA, in Lobosphaera sp. IPPAS C-2047. The immobilization on PEI increased ARA percentage in the total tatty acids and ARA accumulation by 72% and 81%, compared with the suspended cells cultured respectively in complete or nitrogen-deprived medium 14 days. Immobilization of Lobosphaera sp. on the chitosan-based carrier declined ARA but increased oleic and α-linoleic acid percentages. Mechanisms of the effects of immobilization on fatty acid profiles of the microalgae are discussed.

Keywords

microalgae; attached cultivation; chitosan; polyethylenimine; fatty acids; arachidonic acid

Subject

Chemistry and Materials Science, Polymers and Plastics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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