Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isolation and Characterization of Analytical Marker Compound from Polyherbal Antigout Remedy

Version 1 : Received: 8 January 2023 / Approved: 9 January 2023 / Online: 9 January 2023 (07:02:22 CET)

How to cite: Shaukat, A.; Hussain, K. Isolation and Characterization of Analytical Marker Compound from Polyherbal Antigout Remedy. Preprints 2023, 2023010149. https://doi.org/10.20944/preprints202301.0149.v1 Shaukat, A.; Hussain, K. Isolation and Characterization of Analytical Marker Compound from Polyherbal Antigout Remedy. Preprints 2023, 2023010149. https://doi.org/10.20944/preprints202301.0149.v1

Abstract

Objectives: One of the home-made herbal remedy being traditionally used against gout is evaluated to identify any new and characteristic marker compound which may be used as characteristic analytical marker to standardize this polyherbal remedy. Materials and method: A distinct and remarkable peak in the HPLC chromatogram of remedy was identified which was further isolated by fraction collector. This fraction was then evaporated and characterized for structure elucidation using set of various techniques such as RP-HPLC,UV, FTIR, TLC, EIMS and NMR. Results: The compound characterized was non polar compound, ester of betulin and palmitoleic acid. Although the remedy has been standardized using set of four markers as discussed in literature but this newly developed marker compound can be used to further standardize this remedy and other herbal preparations comprising of this marker compound.Conclusion: Hence the isolated compound can be used as novel analytical marker in the standardization of this herbal remedy and the other herbal preparations containing such marker compound

Keywords

Betulin; palmitoleic acid; NMR; EIMS; fragmentation; polyherbal

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

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