Jiménez-Zapata, D.L.; Quiroga-Pérez, M.; Quiroz-Yepes, M.; Marulanda-Tobón, A.; Álvarez, J.C.; Mosquera-López, S. Development of a Method for Detecting and Estimating Moniliophthora roreri Spore Loads Based on Spore Traps and qPCR. J. Fungi2023, 9, 47.
Jiménez-Zapata, D.L.; Quiroga-Pérez, M.; Quiroz-Yepes, M.; Marulanda-Tobón, A.; Álvarez, J.C.; Mosquera-López, S. Development of a Method for Detecting and Estimating Moniliophthora roreri Spore Loads Based on Spore Traps and qPCR. J. Fungi 2023, 9, 47.
Jiménez-Zapata, D.L.; Quiroga-Pérez, M.; Quiroz-Yepes, M.; Marulanda-Tobón, A.; Álvarez, J.C.; Mosquera-López, S. Development of a Method for Detecting and Estimating Moniliophthora roreri Spore Loads Based on Spore Traps and qPCR. J. Fungi2023, 9, 47.
Jiménez-Zapata, D.L.; Quiroga-Pérez, M.; Quiroz-Yepes, M.; Marulanda-Tobón, A.; Álvarez, J.C.; Mosquera-López, S. Development of a Method for Detecting and Estimating Moniliophthora roreri Spore Loads Based on Spore Traps and qPCR. J. Fungi 2023, 9, 47.
Abstract
Frosty pod rot, caused by Moniliophthora roreri, is the most damaging disease of cacao in Latin America. However, to better comprehend its epidemiology, we must understand its dissemination and proliferation. Still, we ignore how loads of M. roreri spores fluctuate across growing seasons since we lack a reliable technique to quantify M. roreri spores in the fields. Therefore, we developed a method that uses a spore trap to capture M. roreri spores and qPCR to quantify them. This study demonstrated that this technique could quantify 3.9 x104 M. roreri spores with a 95 % confidence level. However, it could not differentiate between M. roreri and its close relative, M. perniciosa. Despite this limitation, we could detect and quantify Moniliophthora spores from environmental samples taken from a cacao field. This technique can help the phytopathologist address studies more accurately in disseminating cacao pathogens.
Keywords
spore trap; qPCR; Moniliasis; Theobroma cacao; Frosty pod rot
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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