Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes

Version 1 : Received: 7 July 2022 / Approved: 12 July 2022 / Online: 12 July 2022 (04:04:08 CEST)

How to cite: Calle, A.; Ramírez, M.Á. Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes . Preprints 2022, 2022070167 (doi: 10.20944/preprints202207.0167.v1). Calle, A.; Ramírez, M.Á. Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes . Preprints 2022, 2022070167 (doi: 10.20944/preprints202207.0167.v1).

Abstract

The European mink (Mustela lutreola) is one of Europe's most endangered species, and it is on the brink of extinction in the Iberian Peninsula. The species' precarious situation requires the application of new ex-situ conservation methodologies that complement the existing ex-situ and in-situ conservation measures. Here, we report for the first time the establishment of a biobank of European mink mesenchymal stem cells (emMSC) and oocytes from specimens found dead in the Iberian Peninsula, either free or in captivity. New emMSC lines were isolated from different tissues: bone marrow (emBM-MSC), oral mucosa (emOM-MSc), dermal skin (emDS-MSC), oviduct (emO-MSc), endometrium (emE-MSC), testicular (emT-MSC), and adipose tissue from two different adipose depots: subcutaneous (emSCA-MSC) and ovarian (emOA-MSC). All the eight emMSC lines showed plastic adhesion, detectable expression of characteristic markers of MSCs and, when cultured under osteogenic and adipogenic conditions, differentiation capacity to these lineages. Additionally, we were able to keep 227 Cumulus-oocyte complexes (COCs) in the biobank, 97 of which are grade I or II. The European mink MSC and oocyte biobank will allow for the conservation of the species' genetic variability, the application of assisted reproduction techniques, and the development of in vitro models for studying the molecular mechanisms of infectious diseases that threaten the species' precarious situation.

Keywords

European mink; ex situ conservation; biobank; mesenchymal stem/stromal cells

Subject

LIFE SCIENCES, Cell & Developmental Biology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.

We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.