Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Characterization and In Vivo Toxicity Assay of Uncoated Silicon Nanoparticles

Konstantin V. Zolotarev
* ORCID logo ,
Anton N. Mikhailov
,
Valeriya I. Nakhod
,
Maxim A. Sanzhakov
,
Elena G. Tikhonova
,
Marina V. Mikhailova
Version 1 : Received: 11 May 2022 / Approved: 12 May 2022 / Online: 12 May 2022 (07:39:46 CEST)

How to cite: Zolotarev, K.V.; Mikhailov, A.N.; Nakhod, V.I.; Sanzhakov, M.A.; Tikhonova, E.G.; Mikhailova, M.V. Characterization and In Vivo Toxicity Assay of Uncoated Silicon Nanoparticles. Preprints 2022, 2022050159. https://doi.org/10.20944/preprints202205.0159.v1 Zolotarev, K.V.; Mikhailov, A.N.; Nakhod, V.I.; Sanzhakov, M.A.; Tikhonova, E.G.; Mikhailova, M.V. Characterization and In Vivo Toxicity Assay of Uncoated Silicon Nanoparticles. Preprints 2022, 2022050159. https://doi.org/10.20944/preprints202205.0159.v1

Abstract

Silicon nanoparticles (SiNPs) are highly promising for biological and biomedical applications, including bioimaging, due to their unique opltical properties (i.e. strong fluorescence and very high photostability). Their low or negligible in vitro toxicity has been reported, but in vivo toxicity and biological effects of SiNPs are still uncertain. Uncoated SiNPs were dispersed in distilled water via sonication, and their rapid aggregation was observed (319.0 ± 2.4 nm particle size). In vivo toxicity was studied using Danio rerio embryos and larvae. Rapid aggregation in their incubation medium was observed; besides that, SiNPs at 25 mg/L or higher concentration induced swim bladder malformation and/or death of the fish. The estimated LC50 value for 7-day larvae was 180 mg/L. This is the first in vivo toxicity study of uncoated and unfunctionalized SiNPs. To achieve better stability in biological media and lower toxicity, SiNPs should be covered with hydrophilic layers, but their absorption by cellular membranes may be weaker in this case.

Keywords

uncoated silicon nanoparticles; aggregation; in vivo toxicity; LC50 for Danio rerio larvae

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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