Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Revealed Distinctive Features of Cold Adapted Notothenioidei

Alessia Ametrano
ORCID logo ,
Simona Picchietti
ORCID logo ,
Laura Guerra
,
Stefano Giacomelli
,
Umberto Oreste
,
Maria Rosaria Coscia
*
Version 1 : Received: 15 February 2022 / Approved: 17 February 2022 / Online: 17 February 2022 (11:47:52 CET)
Version 2 : Received: 24 June 2022 / Approved: 27 June 2022 / Online: 27 June 2022 (08:41:00 CEST)

A peer-reviewed article of this Preprint also exists.

Ametrano, A.; Picchietti, S.; Guerra, L.; Giacomelli, S.; Oreste, U.; Coscia, M. R. Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus Bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei. International Journal of Molecular Sciences, 2022, 23, 7783. https://doi.org/10.3390/ijms23147783. Ametrano, A.; Picchietti, S.; Guerra, L.; Giacomelli, S.; Oreste, U.; Coscia, M. R. Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus Bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei. International Journal of Molecular Sciences, 2022, 23, 7783. https://doi.org/10.3390/ijms23147783.

Abstract

Previously, we identified and characterized the IgM and IgT classes in the Antarctic teleost Trematomus bernacchii, a species belonging to the Perciform suborder Notothenoidei. Herein we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared it to pIgR of multiple teleost species belonging to five perciform suborders, including 11 Antarctic and one non-Antarctic (Cottoperca gobio) notothenioid species, the latter living in less cold periantarctic sea. Analysis of T. bernacchii pIgR cDNA unveiled multiple amino acid substitutions unique to Antarctic species, all introducing adaptive features, including N-glycosylation sequons. Interestingly, C. gobio shared most features with the other perciforms rather than with the cold adapted relatives. T. bernacchii pIgR transcripts were predominantly expressed in mucosal tissues, as indicated by q-PCR and in situ hybridization analysis. These results suggest that in cold adapted species pIgR preserved its fundamental role in mucosal immune defense, although remarkable gene structure modifications occurred.

Keywords

pIgR; gene structure; cold environment; gene expression; teleost immunity; adaptive evolution; mucosal tissues; genome alteration; Notothenioidei; IgV domains.

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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