Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Delineating the Tissue-Mediated Drought Stress Governed Tuning of Conserved miR408 and Its Targets in Rice

Version 1 : Received: 7 February 2022 / Approved: 8 February 2022 / Online: 8 February 2022 (13:17:14 CET)

How to cite: Balyan, S.; Kansal, S.; Jajo, R.; Rajiv Behere, P.; Chatterjee, R.; Raghuvanshi, S. Delineating the Tissue-Mediated Drought Stress Governed Tuning of Conserved miR408 and Its Targets in Rice. Preprints 2022, 2022020113 (doi: 10.20944/preprints202202.0113.v1). Balyan, S.; Kansal, S.; Jajo, R.; Rajiv Behere, P.; Chatterjee, R.; Raghuvanshi, S. Delineating the Tissue-Mediated Drought Stress Governed Tuning of Conserved miR408 and Its Targets in Rice. Preprints 2022, 2022020113 (doi: 10.20944/preprints202202.0113.v1).

Abstract

MicroRNAs act as the cardinal post-transcriptional monitors of gene regulatory networks sculpturing the developmental plasticity and stress responses in plants. Single miRNA target several genes and how the transcriptional regulation of miRNA impacts its pool of targets in different tissues and stress conditions is still elusive. The present study investigated the highly conserved and evolving MIR408 family comprehensively by redefining its evolutionary conservation and diversification in plants followed by detailed functional analysis in rice. MIR408 family comprises three dominant mature forms (21 nt) including a distinct monocot variant. Plant MIR408 family can be divided into six groups. miR408 majorly cleave genes belonging to blue copper protein in addition to several other species-specific targets in plants. Screening of 4726 rice accessions identified 22 sequence variants in 1 Kb upstream (15) and MIR408 region leading to the identification of 8 haplotypes (3: Japonica-specific and 5: Indica-specific). miR408-3p follows flag leaf preferential and drought upregulated expression profile in flag leaf and roots of N22 which seems to be regulated by differential fraction of mCs in the precursor region. The active pool of miR408 regulated targets under control and drought conditions is impacted by the tissue type. Comparative expression analysis of miR408/target module under different sets of conditions features 83 targets exhibiting antagonistic expression in rice. Twelve high confidence targets including 4 plantacyanins (OsUCL6, 7, 9 and 30), pirin, OsLPR1, OsCHUP1, OsDOF12, OsBGLU1, glycine rich cell wall, deoxyuridine 5-triphosphate nucleotidohydrolaseand OsERF7 with antagonistic expression under most conditions. Further, over-expression of osa-MIR408 in drought sensitive rice cultivar leads to the massive enhancement of vegetative growth in rice with improved ETR and Y(II) and enhanced the dehydration stress tolerance at seedling stage.

Keywords

miR408; conserved; drought; N22; miRNA target

Subject

BIOLOGY, Plant Sciences

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