Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Using DNA Metabarcoding to Identify Floral Visitation by Pollinators

Version 1 : Received: 28 January 2022 / Approved: 1 February 2022 / Online: 1 February 2022 (16:24:01 CET)

A peer-reviewed article of this Preprint also exists.

Lowe, A.; Jones, L.; Witter, L.; Creer, S.; de Vere, N. Using DNA Metabarcoding to Identify Floral Visitation by Pollinators. Diversity 2022, 14, 236. Lowe, A.; Jones, L.; Witter, L.; Creer, S.; de Vere, N. Using DNA Metabarcoding to Identify Floral Visitation by Pollinators. Diversity 2022, 14, 236.

Journal reference: Diversity 2022, 14, 236
DOI: 10.3390/d14040236

Abstract

The identification of floral visitation by pollinators provides an opportunity to improve our understanding of the fine-scale ecological interactions between plants and pollinators, contributing to biodiversity conservation and promoting ecosystem health. In this review we outline the various methods which can be used to identify floral visitation, providing a comparison between molecular and non-molecular methods. We review the literature covering the ways in which DNA metabarcoding has been used to answer ecological questions relating to plant use by pollinators and discuss the findings of this research. We present detailed methodological considerations for each step of the metabarcoding workflow, from sampling through to amplification and finally bioinformatic analysis. Detailed guidance is provided to researchers for utilization of these techniques, emphasizing the importance of standardization of methods and improving the reliability of results. Future opportunities and directions of using molecular methods to analyze plant-pollinator interactions are then discussed.

Keywords

DNA metabarcoding; pollen; pollinators; pollen metabarcoding; plant-pollinator interactions; eDNA; DNA barcoding, honeybees; bumblebees; hoverflies

Subject

BIOLOGY, Ecology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.