PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Contact Toxicity of Various Stemona Collinsiae Root Extracts Against Periplaneta Americana (Dictyoptera: Blattodea) And Detection of Didehydrostemofoline Distribution in Tissue Using MALDI Imaging Mass Spectrometry
Version 1
: Received: 28 December 2021 / Approved: 30 December 2021 / Online: 30 December 2021 (12:39:41 CET)
How to cite:
Sakulpanich, A.; Ounklong, K.; Sommanat, J.; Phayakkaphon, A.; Srisawat, R.; Ruangsittichai, J. Contact Toxicity of Various Stemona Collinsiae Root Extracts Against Periplaneta Americana (Dictyoptera: Blattodea) And Detection of Didehydrostemofoline Distribution in Tissue Using MALDI Imaging Mass Spectrometry. Preprints2021, 2021120486. https://doi.org/10.20944/preprints202112.0486.v1
Sakulpanich, A.; Ounklong, K.; Sommanat, J.; Phayakkaphon, A.; Srisawat, R.; Ruangsittichai, J. Contact Toxicity of Various Stemona Collinsiae Root Extracts Against Periplaneta Americana (Dictyoptera: Blattodea) And Detection of Didehydrostemofoline Distribution in Tissue Using MALDI Imaging Mass Spectrometry. Preprints 2021, 2021120486. https://doi.org/10.20944/preprints202112.0486.v1
Sakulpanich, A.; Ounklong, K.; Sommanat, J.; Phayakkaphon, A.; Srisawat, R.; Ruangsittichai, J. Contact Toxicity of Various Stemona Collinsiae Root Extracts Against Periplaneta Americana (Dictyoptera: Blattodea) And Detection of Didehydrostemofoline Distribution in Tissue Using MALDI Imaging Mass Spectrometry. Preprints2021, 2021120486. https://doi.org/10.20944/preprints202112.0486.v1
APA Style
Sakulpanich, A., Ounklong, K., Sommanat, J., Phayakkaphon, A., Srisawat, R., & Ruangsittichai, J. (2021). Contact Toxicity of Various Stemona Collinsiae Root Extracts Against Periplaneta Americana (Dictyoptera: Blattodea) And Detection of Didehydrostemofoline Distribution in Tissue Using MALDI Imaging Mass Spectrometry. Preprints. https://doi.org/10.20944/preprints202112.0486.v1
Chicago/Turabian Style
Sakulpanich, A., Raweewan Srisawat and Jiraporn Ruangsittichai. 2021 "Contact Toxicity of Various Stemona Collinsiae Root Extracts Against Periplaneta Americana (Dictyoptera: Blattodea) And Detection of Didehydrostemofoline Distribution in Tissue Using MALDI Imaging Mass Spectrometry" Preprints. https://doi.org/10.20944/preprints202112.0486.v1
Abstract
Contact toxicity against Periplaneta americana has never been tested with S. collinsiae root extract. Hexane, dichloromethane, ethanol and water extracts were tested in final-instar nymphs and adult P. americana by topical application method. The dichloromethane extract showed the high-est potency of contact toxicity against the final-instar nymphs (41-100% corrected mortality at 48 hours), lowest LC50 (1.5±0.2 %w/v at 48 hours), and lowest LT50 (36.1±0.8 hours at 10%w/v) while the water crude extract lacked the contact toxicity (0-0% corrected mortality at 48 hours). Signs of toxicity, such as excited movement, trembling body, motionlessness, and swollen abdomen segment including irregularly extended foregut were found at the both stages of P. americana dropping with solutions of dichloromethane extract. Detection of didehydrostemofoline distri-bution using IMS revealed that didehydrostemofoline distributed in the tissue of the dead fi-nal-instar nymph and adult P. americana contacting with dichloromethane extract, but it was not found in tissue of euthanized P. americana which exposed to the water extract. Didehydrostemo-foline in the extract was a cause of toxicity signs and death of P. americana via a contact route. Thus, dichloromethane extract and didehydrostemofoline could be used as an active ingredient and chemical marker in aerosol and spray formulations for cockroach control.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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