Submitted:
19 November 2021
Posted:
22 November 2021
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Abstract
Cesiumtrifluoroacetate (CsTFA) is a key chemical for RNA-based stable isotope analyses to link the structure and function of microbial communities. We report a protocol to easily synthesize CsTFA from Cesiumcarbonate (Cs2CO3) and Trifluoroacetate (TFA) and show that self-synthesized CsTFA behaves similar to commercial CsTFA in the separation of isotopically labelled and unlabelled E. coli RNA.
Keywords:
Stable Isotope Probing
; RNA
; density gradient
; ultracentrifugation
; microbial ecology
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