Preprint Short Note Version 1 Preserved in Portico This version is not peer-reviewed

Do It Yourself! – Experiences with Self-Synthesized CsTFA for RNA-SIP Analyses

Version 1 : Received: 19 November 2021 / Approved: 22 November 2021 / Online: 22 November 2021 (10:41:51 CET)

A peer-reviewed article of this Preprint also exists.

Severin Weis, Susanne Jacksch, Marius Welkerling, Magnus S. Schmidt, Markus Egert, Do it yourself! – Initial experiences with self-synthesized CsTFA for RNA-SIP analyses, Journal of Microbiological Methods, Volume 194, 2022, 106432, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2022.106432. Severin Weis, Susanne Jacksch, Marius Welkerling, Magnus S. Schmidt, Markus Egert, Do it yourself! – Initial experiences with self-synthesized CsTFA for RNA-SIP analyses, Journal of Microbiological Methods, Volume 194, 2022, 106432, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2022.106432.

Abstract

Cesiumtrifluoroacetate (CsTFA) is a key chemical for RNA-based stable isotope analyses to link the structure and function of microbial communities. We report a protocol to easily synthesize CsTFA from Cesiumcarbonate (Cs2CO3) and Trifluoroacetate (TFA) and show that self-synthesized CsTFA behaves similar to commercial CsTFA in the separation of isotopically labelled and unlabelled E. coli RNA.

Keywords

Stable Isotope Probing; RNA; density gradient; ultracentrifugation; microbial ecology

Subject

Biology and Life Sciences, Immunology and Microbiology

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