Severin Weis, Susanne Jacksch, Marius Welkerling, Magnus S. Schmidt, Markus Egert,
Do it yourself! – Initial experiences with self-synthesized CsTFA for RNA-SIP analyses,
Journal of Microbiological Methods,
Volume 194,
2022,
106432,
ISSN 0167-7012,
https://doi.org/10.1016/j.mimet.2022.106432.
Severin Weis, Susanne Jacksch, Marius Welkerling, Magnus S. Schmidt, Markus Egert,
Do it yourself! – Initial experiences with self-synthesized CsTFA for RNA-SIP analyses,
Journal of Microbiological Methods,
Volume 194,
2022,
106432,
ISSN 0167-7012,
https://doi.org/10.1016/j.mimet.2022.106432.
Severin Weis, Susanne Jacksch, Marius Welkerling, Magnus S. Schmidt, Markus Egert,
Do it yourself! – Initial experiences with self-synthesized CsTFA for RNA-SIP analyses,
Journal of Microbiological Methods,
Volume 194,
2022,
106432,
ISSN 0167-7012,
https://doi.org/10.1016/j.mimet.2022.106432.
Severin Weis, Susanne Jacksch, Marius Welkerling, Magnus S. Schmidt, Markus Egert,
Do it yourself! – Initial experiences with self-synthesized CsTFA for RNA-SIP analyses,
Journal of Microbiological Methods,
Volume 194,
2022,
106432,
ISSN 0167-7012,
https://doi.org/10.1016/j.mimet.2022.106432.
Abstract
Cesiumtrifluoroacetate (CsTFA) is a key chemical for RNA-based stable isotope analyses to link the structure and function of microbial communities. We report a protocol to easily synthesize CsTFA from Cesiumcarbonate (Cs2CO3) and Trifluoroacetate (TFA) and show that self-synthesized CsTFA behaves similar to commercial CsTFA in the separation of isotopically labelled and unlabelled E. coli RNA.
Keywords
Stable Isotope Probing; RNA; density gradient; ultracentrifugation; microbial ecology
Subject
LIFE SCIENCES, Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
