Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Bioassay-guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-resistant Staphylococcus aureus

Version 1 : Received: 9 September 2021 / Approved: 13 September 2021 / Online: 13 September 2021 (07:21:28 CEST)

A peer-reviewed article of this Preprint also exists.

Muhammad, S.L.; Wada, Y.; Mohammed, M.; Ibrahim, S.; Musa, K.Y.; Olonitola, O.S.; Ahmad, M.H.; Mustapha, S.; Abdul Rahman, Z.; Sha’aban, A. Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus. Appl. Microbiol. 2021, 1, 520-536. Muhammad, S.L.; Wada, Y.; Mohammed, M.; Ibrahim, S.; Musa, K.Y.; Olonitola, O.S.; Ahmad, M.H.; Mustapha, S.; Abdul Rahman, Z.; Sha’aban, A. Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus. Appl. Microbiol. 2021, 1, 520-536.

Journal reference: Appl. Microbiol. 2021, 1, 34
DOI: 10.3390/applmicrobiol1030034

Abstract

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.

Keywords

bioassay; chromatography; folkloric medicine; methicillin-resistant Staphylococcus aureus; Senna alata; spectroscopy

Subject

LIFE SCIENCES, Microbiology

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