Working Paper Article Version 2 This version is not peer-reviewed

Versatile Cell and Animal Models for Advanced Investigation of Lead Poisoning

Version 1 : Received: 23 August 2021 / Approved: 25 August 2021 / Online: 25 August 2021 (10:39:42 CEST)
Version 2 : Received: 17 September 2021 / Approved: 17 September 2021 / Online: 17 September 2021 (11:58:04 CEST)
Version 3 : Received: 1 October 2021 / Approved: 1 October 2021 / Online: 1 October 2021 (11:12:13 CEST)

A peer-reviewed article of this Preprint also exists.

Yang, D.-M.; Chang, Y.-F. Versatile Cell and Animal Models for Advanced Investigation of Lead Poisoning. Biosensors 2021, 11, 371. Yang, D.-M.; Chang, Y.-F. Versatile Cell and Animal Models for Advanced Investigation of Lead Poisoning. Biosensors 2021, 11, 371.


The heavy metal lead (Pb) can irreversibly damage the human nervous system. To help understand Pb-induced damage, we applied a genetically encoded Förster resonance energy transfer (FRET)-based Pb biosensor Met-lead 1.44 M1 to two living systems to monitor the concentration of Pb: induced pluripotent stem cell (iPSC)-derived cardiomyocytes as a semi-tissue platform, and Drosophila melanogaster fruit flies as an in vivo animal model. Different FRET imaging modalities were used to obtain FRET signals, which represented the presence of Pb in the tested samples in different spatial dimensions. In iPSC-derived cardiomyocytes, the relationship between beating activity (20-24 beats per minute, bpm) determined from the fluctuation of fluorescent signals and the concentrations of Pb represented by the FRET emission ratio values of Met-lead 1.44 M1 are simultaneously revealed. Pb (50 μM) impacted the beating, and two drugs that can stop the Pb entry differentially influenced the beating activity, i.e. verapamil (2 μM) can’t reverse while 2-APB (50 μM) partially remained (16 bpm). The results clearly demonstrate a potential of this biosensor system as an anti-Pb drug screening application. In the Drosophila model, Pb was detected within the adult brain or larval central nervous system (Cha-gal4>UAS-Met-lead 1.44 M1) using fast epifluorescence and high-resolution two-photon 3D FRET ratio image systems. The tissue-specific expression of Pb biosensor provides a great opportunity to explore the possible Pb-specific populations within living organisms. We believe that this integrated Pb biosensor system can be applied to the prevention of Pb poisoning and advanced research on Pb neurotoxicology.


Drosophila melanogaster; fluorescence resonance energy transfer; Met-lead 1.44 M1; Pb biosensor


Biology and Life Sciences, Biology and Biotechnology

Comments (1)

Comment 1
Received: 17 September 2021
Commenter: De-Ming Yang
Commenter's Conflict of Interests: Author
Comment: Abstract modified
Introduction modified
Materials and Methods add pretreatment of Pb and drugs
Results, Figure 1C, 1D, Figure S1-S4 added
Figures add scale bars, treatments
Table 1 modified beeating frequency 
Discussion modified 
Conclusion modified
References add three new and modified sequence
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