Wang, X.; Liu, D.; Lin, J.; Zhu, T.; Liu, N.; Yang, X.; Ma, J.; Sui, S. Carotenoid Cleavage Dioxygenase Genes of Chimonanthus praecox, CpCCD7 and CpCCD8, Regulate Shoot Branching in Arabidopsis. Preprints2021, 2021080308. https://doi.org/10.20944/preprints202108.0308.v1
Wang, X., Liu, D., Lin, J., Zhu, T., Liu, N., Yang, X., Ma, J., & Sui, S. (2021). Carotenoid Cleavage Dioxygenase Genes of <em>Chimonanthus praecox</em>, <em>CpCCD7</em> and <em>CpCCD8</em>, Regulate Shoot Branching in <em>Arabidopsis</em>. Preprints. https://doi.org/10.20944/preprints202108.0308.v1
Wang, X., Jing Ma and Shunzhao Sui. 2021 "Carotenoid Cleavage Dioxygenase Genes of <em>Chimonanthus praecox</em>, <em>CpCCD7</em> and <em>CpCCD8</em>, Regulate Shoot Branching in <em>Arabidopsis</em>" Preprints. https://doi.org/10.20944/preprints202108.0308.v1
Strigolactones (SLs) regulate plant shoot development by inhibiting axillary bud growth and branching. However, the role of SLs in wintersweet (Chimonanthus praecox) shoot branching remains unknown. Here, we identified and isolated two wintersweet genes, CCD7 and CCD8, in-volved in the SL biosynthetic pathway. Quantitative real-time PCR revealed that CpCCD7 and CpCCD8 were down-regulated in wintersweet during branching. When new shoots were formed, expression levels of CpCCD7 and CpCCD8 were almost the same as the control (un-decapitation). CpCCD7 was expressed in all tissues, with the highest expression in shoot tips and roots, while CpCCD8 showed the highest expression in roots. Both CpCCD7 and CpCCD8 localized to chloroplasts in Arabidopsis. CpCCD7 and CpCCD8 overexpression restored the phenotypes of branching mutant max3-9 and max4-1, respectively. CpCCD7 overexpression reduced the rosette branch number, whereas CpCCD8 overexpression lines showed no phenotypic differences compared with wild-type plants. Additionally, the expression of AtBRC1 was significantly up-regulated in transgenic lines, indicating that two CpCCD genes functioned similarly to the homologous genes of the Arabidopsis. Overall, our study demonstrates that CpCCD7 and CpCCD8 exhibit conserved functions in the CCD pathway, which controls shoot development in wintersweet. This research provides a molecular and theoretical basis for further understanding branch development in wintersweet.
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