Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

CRISPR-Cas. A Revolution in the Treatment and Study of ESKAPE Infections: Pre-Clinical Studies

Version 1 : Received: 24 May 2021 / Approved: 25 May 2021 / Online: 25 May 2021 (09:58:47 CEST)

How to cite: Gonzalez de Aledo, M.; Gonzalez-Bardanca, M.; Blasco, L.; Pacios, O.; Bleriot, I.; Fernandez-Garcia, L.; López, M.; Fernández-Quejo, M.; Bou, G.; Tomás, M. CRISPR-Cas. A Revolution in the Treatment and Study of ESKAPE Infections: Pre-Clinical Studies. Preprints 2021, 2021050598 (doi: 10.20944/preprints202105.0598.v1). Gonzalez de Aledo, M.; Gonzalez-Bardanca, M.; Blasco, L.; Pacios, O.; Bleriot, I.; Fernandez-Garcia, L.; López, M.; Fernández-Quejo, M.; Bou, G.; Tomás, M. CRISPR-Cas. A Revolution in the Treatment and Study of ESKAPE Infections: Pre-Clinical Studies. Preprints 2021, 2021050598 (doi: 10.20944/preprints202105.0598.v1).

Abstract

One of the biggest threats we face globally is the emergence of antimicrobial resistant (AMR) bacteria, which runs in parallel with a lack in the development of new antimicrobials. Among these AMR bacteria, pathogens belonging to the ESKAPE group can be highlighted (Enterococcus spp, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp) due to their profile of drug resistance and virulence. Therefore, innovative lines of treatment must be developed for these bacteria. In this review, we summarize the different strategies for the treatment and study of molecular mechanisms of AMR in the ESKAPE pathogens based on the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) proteins' technologies: loss of plasmid or cellular viability, random mutation or gene deletion as well directed mutations that lead to a gene's loss of function.

Subject Areas

CRISPR-Cas; ESKAPE pathogens; treatment

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