Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Whether Supercritical Extracts from the Aerial Parts of Helianthus salicifolius and Helianthus tuberosus May be Regarded as a Potential Raw Materials for Non-bioenergy Purposes?

Anna Malm
ORCID logo ,
Agnieszka Grzegorczyk
,
Anna Biernasiuk
* ORCID logo ,
Tomasz Baj
,
Edward Rój
,
Katarzyna Tyśkiewicz
,
Agnieszka Dębczak
,
Mariusz Jerzy Stolarski
ORCID logo ,
Michał Krzyżaniak
ORCID logo ,
Ewelina Olba-Zięty
ORCID logo
Version 1 : Received: 31 October 2020 / Approved: 2 November 2020 / Online: 2 November 2020 (11:47:35 CET)

A peer-reviewed article of this Preprint also exists.

Malm, A.; Grzegorczyk, A.; Biernasiuk, A.; Baj, T.; Rój, E.; Tyśkiewicz, K.; Dębczak, A.; Stolarski, M.J.; Krzyżaniak, M.; Olba-Zięty, E. Could Supercritical Extracts from the Aerial Parts of Helianthus salicifolius A. Dietr. and Helianthus tuberosus L. Be Regarded as Potential Raw Materials for Biocidal Purposes? Agriculture 2021, 11, 10. Malm, A.; Grzegorczyk, A.; Biernasiuk, A.; Baj, T.; Rój, E.; Tyśkiewicz, K.; Dębczak, A.; Stolarski, M.J.; Krzyżaniak, M.; Olba-Zięty, E. Could Supercritical Extracts from the Aerial Parts of Helianthus salicifolius A. Dietr. and Helianthus tuberosus L. Be Regarded as Potential Raw Materials for Biocidal Purposes? Agriculture 2021, 11, 10.

Abstract

The extracts from the aerial parts of Helianthus salicifolius A. Dietr and Helianthus tuberosus L. collected in June were obtained using carbon dioxide supercritical fluid extraction with water as co-solvent. The antimicrobial activity in vitro of these extracts were determined against the reference species of Gram-positive and Gram-negative bacteria as well as fungi, representing by the yeast species of Candida spp. The following parameters were estimated: minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC). Both extracts were found to possess antimicrobial activity with MIC = 0.62-5 mg mL-1 for bacteria and MIC = 5-10 mg mL-1 for yeasts, showing bactericidal (MBC/MIC = 2-4) or fungicidal effect (MFC/MIC = 1-2 ). The highest activity was observed against S. aureus ATCC 29213 (MIC = 0.62 mg mL-1 for H. salicifolius extract; MIC = 2.5 mg mL-1 for H. tuberosus extract). Bactericidal effect of both extracts against S. aureus ATCC 29213 was confirmed by time-kill assay. Higher antioxidant activity was found for H. tuberosus extract (EC50 = 0.332 mg mL-1) as compared to that of H. salicifolius (EC50 = 0.609 mg mL-1). The total polyphenol content (TPC) expressed as gallic acid equivalents (GAE) was 13.75 ± 0.50 mg GAE (g of H. salicifolius extract)-1 and 33.06 ± 0.80 mg GAE (g of H. tuberosus extract)-1. There was a correlation between the antioxidant potential of both extracts and TPC but not between antistaphylococcal activity and TPC. The obtained data suggest potential application of these extracts as the natural preparations with the biocidal activity, including those with antistaphylococcal activity. Besides, both extracts may be regarded as potential natural conservants in cosmetics as well as natural preservatives in food.

Keywords

willow-leaf sunflower; Jerusalem artichoke; supercritical extraction; water as co-solvent; antimicrobial activity; biocidal effect

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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