Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Phytochemical, Biological and Computational Investigations of Erythrina fusca Lour. to Assess Antimalarial Property against Plasmodium falciparum

Saiful Arefeen Sazed
* ORCID logo ,
Ohedul Islam
,
Sarah L. Bliese
,
Muhammad Riadul Haque Hossainey
,
Mahfuza Afroz Soma
,
Mohammad Abdur Rashid
,
Mohammad Sharifur Rahman
,
Mohammad Shafiul Alam
ORCID logo
Version 1 : Received: 27 October 2020 / Approved: 28 October 2020 / Online: 28 October 2020 (09:59:54 CET)
Version 2 : Received: 21 October 2021 / Approved: 21 October 2021 / Online: 21 October 2021 (12:08:52 CEST)

How to cite: Sazed, S.A.; Islam, O.; Bliese, S.L.; Hossainey, M.R.H.; Soma, M.A.; Rashid, M.A.; Rahman, M.S.; Alam, M.S. Phytochemical, Biological and Computational Investigations of Erythrina fusca Lour. to Assess Antimalarial Property against Plasmodium falciparum. Preprints 2020, 2020100576. https://doi.org/10.20944/preprints202010.0576.v1 Sazed, S.A.; Islam, O.; Bliese, S.L.; Hossainey, M.R.H.; Soma, M.A.; Rashid, M.A.; Rahman, M.S.; Alam, M.S. Phytochemical, Biological and Computational Investigations of Erythrina fusca Lour. to Assess Antimalarial Property against Plasmodium falciparum. Preprints 2020, 2020100576. https://doi.org/10.20944/preprints202010.0576.v1

Abstract

For centuries medicinal plants have been traditionally used for prophylaxis and ailment of diseases. Nowadays it’s easy to isolate, purify, and characterize bioactive compounds with high efficacy. To investigate the medicinal especially antimalarial property of traditionally used plants, a number of Erythrina spp have been reviewed systematically where Erythrina fusca has been selected for further analysis. Phytochemical investigation included chromatographic separation and purification of compounds followed by characterization using NMR. In-vitro antimalarial drug sensitivity ELISA was carried out against chloroquine (CQ) sensitive 3D7 and resistant Dd2 strains. Additional biological tests such as central and peripheral analgesic, antioxidant, anti-diarrheal, hypoglycemic, thrombolytic, and membrane stabilization activities were also investigated. Molecular docking was performed using the isolated compounds against clinically important 14 Plasmodium falciparum proteins. For the first time, Phaseolin, Phytol, β-amyrin, Lupeol, and Stigmasterol are reported here and extracts showed significant antimalarial activity against 3D7 and Dd2 strains (IC50 4.94-22 µg/mL). Potent central analgesic, antioxidant and anti-diarrheal activities (p<0.05) and mild thrombolytic and membrane stabilization properties were also observed. Molecular docking of Phaseolin bolsters its potential as a new antimalarial drug candidate. This study projects significant medicinal values and necessitates further investigations to reveal its potential as a novel source of therapeutics.

Keywords

Antimalarial; Erythrina fusca; Phaseolin; Molecular docking; Phytochemical analysis

Subject

Chemistry and Materials Science, Analytical Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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