Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

A Diverse Virome of Leafroll-Infected Grapevine Unveil by dsRNA Sequencing

Version 1 : Received: 25 September 2020 / Approved: 26 September 2020 / Online: 26 September 2020 (16:03:31 CEST)
Version 2 : Received: 28 September 2020 / Approved: 29 September 2020 / Online: 29 September 2020 (08:49:24 CEST)

A peer-reviewed article of this Preprint also exists.

Fall, M.L.; Xu, D.; Lemoyne, P.; Moussa, I.E.B.; Beaulieu, C.; Carisse, O. A Diverse Virome of Leafroll-Infected Grapevine Unveiled by dsRNA Sequencing. Viruses 2020, 12, 1142. Fall, M.L.; Xu, D.; Lemoyne, P.; Moussa, I.E.B.; Beaulieu, C.; Carisse, O. A Diverse Virome of Leafroll-Infected Grapevine Unveiled by dsRNA Sequencing. Viruses 2020, 12, 1142.

Journal reference: Viruses 2020, 12, 1142
DOI: 10.3390/v12101142

Abstract

Quebec is the third-largest wine grape producing province in Canada, and the industry is constantly expanding. Traditionally, 90% of the grapevine cultivars grown in Quebec were rustic or semi-rustic and largely dominated by winter hardy interspecific hybrid Vitis sp. cultivars. Over the years, the winter protection techniques adopted by growers and climate changes have offered an opportunity to establish V. vinifera L. cultivars (e.g. Pinot Noir). We characterized the virome of leafroll-infected interspecific hybrid cultivar and compared it to the virome of V. vinifera cultivar to support and facilitate the transition of the industry. A dsRNA sequencing method was used to sequence symptomatic and asymptomatic grapevine leaves of different cultivars. The results suggested a complex virome in terms of composition, abundance, richness, and phylogenetic diversity. Three viruses, grapevine Rupestris stem pitting-associated virus, grapevine leafroll-associated virus (GLR) 3 and 2 and hop stunt viroid (HSVd) largely dominated the virome. However, their presence and abundance varied among grapevine cultivars. The symptomless grapevine cultivar Vidal was frequently infected by multiple virus and viroid species and different strains of the same virus, including GLR virus 3 and 2. Our data shows that viruses and viroids associated with the highest number of grapevines expressing symptoms included HSVd, GLR3 and GLR2, in gradient order. However, co-occurrence analysis revealed that the presence of GLR species was randomly associated with the development of virus-like symptoms. These findings and their implication for grapevine leafroll disease management were discussed.

Subject Areas

Grapevine leafroll disease; viromics; dsRNA extraction; virus epidemiology; virus co-occurrence; Vitis vinifera; interspecific hybrid

Comments (1)

Comment 1
Received: 29 September 2020
Commenter: Mamadou Fall
Commenter's Conflict of Interests: Author
Comment: Information hilighted in yellow in previous version was updated. Thank you for considering this version. 
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