Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Microbiome Management by Biological and Chemical Treatments in Maize is Linked to Plant Health

Version 1 : Received: 19 August 2020 / Approved: 21 August 2020 / Online: 21 August 2020 (05:10:34 CEST)

How to cite: Kusstatscher, P.; Wicaksono, W.A.; Thenappan, D.P.; Adam, E.; Müller, H.; Berg, G. Microbiome Management by Biological and Chemical Treatments in Maize is Linked to Plant Health. Preprints 2020, 2020080471 (doi: 10.20944/preprints202008.0471.v1). Kusstatscher, P.; Wicaksono, W.A.; Thenappan, D.P.; Adam, E.; Müller, H.; Berg, G. Microbiome Management by Biological and Chemical Treatments in Maize is Linked to Plant Health. Preprints 2020, 2020080471 (doi: 10.20944/preprints202008.0471.v1).

Abstract

The targeted application of plant growth promoting rhizobacteria (PGPR) provides the key for a future sustainable agriculture with reduced pesticide application. PGPR interaction with the indigenous microbiota is poorly understood but essential to develop reliable applications. Therefore, Stenotrophomonas rhizophila SPA-P69 was applied as seed coating and in combination with a fungicide based on the active ingredients fludioxonil, metalaxyl-M, captan and ziram. Plant performance and rhizosphere composition of treated and non-treated maize plants of two field trials were analyzed. Plant health was significantly increased by treatment; however overall corn yield was not changed. By applying high-throughput amplicon sequencing of the 16S rRNA and the ITS genes, the bacterial and fungal changes in the rhizosphere due to different treatments were determined. Despite treatments had a significant impact on the rhizosphere microbiota (9- 12%), the field site was identified as main driver (27- 37%). Soil microbiota composition from each site was significantly different, which explains the site-specific effects. In this study we were able to show first indications how PGPR treatments increase plant health via microbiome shifts in a site-specific manner. This way first steps towards a detailed understanding of PGPRs and developments of consistently efficient applications in diverse environments are set.

Subject Areas

Zea mays; maize; corn; rhizosphere; 16S rRNA gene; ITS; fungicide; plant growth promoting rhizobacteria

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