Preprint Article Version 1 This version is not peer-reviewed

Comparative Transcriptome Analysis of Two Contrasting Kiwifruit (Actinidia) Genotypes under Waterlogging Stress

Version 1 : Received: 16 July 2020 / Approved: 17 July 2020 / Online: 17 July 2020 (15:40:11 CEST)

How to cite: Li, Z.; Zhong, Y.; Bai, D.; Abid, M.; Zhang, Y.; Lin, M.; Qi, X.; Hu, C.; Fang, J. Comparative Transcriptome Analysis of Two Contrasting Kiwifruit (Actinidia) Genotypes under Waterlogging Stress. Preprints 2020, 2020070395 (doi: 10.20944/preprints202007.0395.v1). Li, Z.; Zhong, Y.; Bai, D.; Abid, M.; Zhang, Y.; Lin, M.; Qi, X.; Hu, C.; Fang, J. Comparative Transcriptome Analysis of Two Contrasting Kiwifruit (Actinidia) Genotypes under Waterlogging Stress. Preprints 2020, 2020070395 (doi: 10.20944/preprints202007.0395.v1).

Abstract

Kiwifruit vines are generally sensitive to waterlogging stress. So far, molecular responses of different kiwifruit genotypes for waterlogging stress are less well-explored. In this study, using RNA-sequencing, we examined transcriptional regulation in the roots of a waterlogging-tolerant genotype KR5 (Actinidia valvata), and a sensitive genotype ‘Hayward’ (Actinidia deliciosa) subjected to 0, 12, 24, and 72 h of waterlogging. Compared with 0 h, transcriptional adjustments of these two genotypes occurred as early as 12 h and became notably pronounced 72 h after waterlogging. Waterlogging stress for 72 h promoted the expression of genes involved in ethylene biosynthesis, sucrose and hexose transport, anaerobic fermentation, nitrate reduction, alanine accumulation, and reactive oxygen scavenging in both genotypes. The differential regulation of genes encoding 9-cis-epoxycarotenoid dioxygenase, phosphoglucomutase, alanine-glyoxylate transaminase, and other enzymes pointed to their diverse strategies upon waterlogging in these two genotypes. In addition, more sucrose and trehalose contents, as well as a higher activity of alcohol dehydrogenase and manganese superoxide dismutases were stimulated in KR5 roots after 72h of waterlogging than that in ‘Hayward’. Overall, our results provided more insights into the molecular basis of the waterlogging response in kiwifruit.

Subject Areas

Actinidia; waterlogging; RNA-sequencing; transcriptional adjustment

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.