Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Spatial Expression Pattern of the Major Ca2+-buffer Proteins in the Mouse Retinal Ganglion Cells

Tamás Kovács-Öller
* ORCID logo ,
Gergely Szarka
,
Ádám J Tengölics
,
Alma Ganczer
,
Boglárka Balogh
,
Edina Szabó-Meleg
,
Miklós Nyitrai
,
Béla Völgyi
ORCID logo
Version 1 : Received: 10 February 2020 / Approved: 11 February 2020 / Online: 11 February 2020 (11:43:03 CET)

A peer-reviewed article of this Preprint also exists.

Kovács-Öller, T.; Szarka, G.; Tengölics, Á.J.; Ganczer, A.; Balogh, B.; Szabó-Meleg, E.; Nyitrai, M.; Völgyi, B. Spatial Expression Pattern of the Major Ca2+-Buffer Proteins in Mouse Retinal Ganglion Cells. Cells 2020, 9, 792. Kovács-Öller, T.; Szarka, G.; Tengölics, Á.J.; Ganczer, A.; Balogh, B.; Szabó-Meleg, E.; Nyitrai, M.; Völgyi, B. Spatial Expression Pattern of the Major Ca2+-Buffer Proteins in Mouse Retinal Ganglion Cells. Cells 2020, 9, 792.

Abstract

The most prevalent Ca2+-buffer proteins (CaBPs: parvalbumin—PV; calbindin—CaB; calretinin—CaR) are widely expressed by various neurons throughout the brain, including the retinal ganglion cells (RGCs). Even though their retinal expression has been extensively studied, a coherent assessment of topographical variations is missing. To examine this, we performed immunohistochemistry (IHC) in the mouse retina. We found variability in the expression levels and cell numbers for CaR, with stronger and more numerous labels in the dorso-central area. CaBP+ cells contributed to RGCs with all soma sizes, indicating heterogeneity. We separated 4-9 RGC clusters in each area based on expression levels and soma sizes. Besides the overall high variety in cluster number and size, the peripheral half of the temporal retina showed the greatest cluster number, indicating a better separation of RGC subtypes there. Multiple labels showed that 39% of the RGCs showed positivity for a single CaBP, 30% expressed two CaBPs, 25% showed no CaBP expression and 6% expressed all three proteins. Finally, we observed an inverse relation between CaB and CaR expression levels in CaB/CaR dually- and CaB/CaR/PV triple labeled RGCs, suggesting a mutual complementary function.

Keywords

parvalbumin; calretinin; calbindin; expression; retina; topography; ganglion cell; calcium buffer protein

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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