Preprint Article Version 1 This version is not peer-reviewed

Nile Tilapia (Oreochromis niloticus) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence

Version 1 : Received: 22 November 2019 / Approved: 24 November 2019 / Online: 24 November 2019 (14:11:40 CET)

How to cite: Osman, K.M.; da Silva Pires, Á.; Luiz Franco, O.; Orabi, A.; Ali, A.H.; Hamada, M.; Alzaben, F.A.; Almuzaini, A.M.; Elbehiry, A. Nile Tilapia (Oreochromis niloticus) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence. Preprints 2019, 2019110282 (doi: 10.20944/preprints201911.0282.v1). Osman, K.M.; da Silva Pires, Á.; Luiz Franco, O.; Orabi, A.; Ali, A.H.; Hamada, M.; Alzaben, F.A.; Almuzaini, A.M.; Elbehiry, A. Nile Tilapia (Oreochromis niloticus) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence. Preprints 2019, 2019110282 (doi: 10.20944/preprints201911.0282.v1).

Abstract

Pseudomonas aeruginosa (P. aeruginosa) produces a suite of virulence factors that are coordinated by Quorum Sensing (QS) contributing to its disease-causing ability in aquaculture. The present study is first of its kind to obtain information regarding the presence and distribution of five QS genes, three virulence genes viz: lasI, lasR, rhlI, rhlR, rhlAB, toxA, aprA and plcH and seven of the Extended-spectrum βlactamases (blaVEB, blaPER, blaTEM,, blaSHV, blaCTX-M1, blaCTX-M2 and blaCTX-M3) of Pseudomonas species isolated from fish meat by direct PCR. Bacterial identification was based mainly on conventional biochemical techniques using the Vitek 2, automated system. Phenotypic sensitivity of antibiotics was established by the agar disc diffusion technique through 16 various antimicrobial drugs. Quantification of their in vitro production of numerous virulence genes outside the cell that are QS dependent namely, pyocyanin, elastase, alkaline protease, biofilm and cytotoxicity of Vero cell was as well executed. Fifteen genes demonstrated an enormous variety in their association. The total number of Pseudomonas species isolates were 30/100 to be identified by the API 20NE system as P. aeruginosa 12/30 (40%), P. fluorescens 8/30 (27%), P. putida 6/30 (20%) and P. alkylphenolia 4/30 (13%). The outcomes of this study have great significance for the strategic designation of QS quenching.

Subject Areas

Nile tilapia; pseudomonas; antibiotic resistance; biofilm formation; virulence genes

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.