PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Nile Tilapia (Oreochromis niloticus) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence
Version 1
: Received: 22 November 2019 / Approved: 24 November 2019 / Online: 24 November 2019 (14:11:40 CET)
How to cite:
Osman, K.M.; da Silva Pires, Á.; Luiz Franco, O.; Orabi, A.; Ali, A.H.; Hamada, M.; Alzaben, F.A.; Almuzaini, A.M.; Elbehiry, A. Nile Tilapia (Oreochromis niloticus) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence. Preprints2019, 2019110282. https://doi.org/10.20944/preprints201911.0282.v1
Osman, K.M.; da Silva Pires, Á.; Luiz Franco, O.; Orabi, A.; Ali, A.H.; Hamada, M.; Alzaben, F.A.; Almuzaini, A.M.; Elbehiry, A. Nile Tilapia (Oreochromis niloticus) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence. Preprints 2019, 2019110282. https://doi.org/10.20944/preprints201911.0282.v1
Osman, K.M.; da Silva Pires, Á.; Luiz Franco, O.; Orabi, A.; Ali, A.H.; Hamada, M.; Alzaben, F.A.; Almuzaini, A.M.; Elbehiry, A. Nile Tilapia (Oreochromis niloticus) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence. Preprints2019, 2019110282. https://doi.org/10.20944/preprints201911.0282.v1
APA Style
Osman, K.M., da Silva Pires, Á., Luiz Franco, O., Orabi, A., Ali, A.H., Hamada, M., Alzaben, F.A., Almuzaini, A.M., & Elbehiry, A. (2019). Nile Tilapia (<em>Oreochromis niloticus</em>) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence. Preprints. https://doi.org/10.20944/preprints201911.0282.v1
Chicago/Turabian Style
Osman, K.M., Abdulaziz M. Almuzaini and Ayman Elbehiry. 2019 "Nile Tilapia (<em>Oreochromis niloticus</em>) as an Aquatic Vector for Pseudomonas Species: Quorum Sensing Association with Antibiotic Resistance, Biofilm Formation and Virulence" Preprints. https://doi.org/10.20944/preprints201911.0282.v1
Abstract
Pseudomonas aeruginosa (P. aeruginosa) produces a suite of virulence factors that are coordinated by Quorum Sensing (QS) contributing to its disease-causing ability in aquaculture. The present study is first of its kind to obtain information regarding the presence and distribution of five QS genes, three virulence genes viz: lasI, lasR, rhlI, rhlR, rhlAB, toxA, aprA and plcH and seven of the Extended-spectrum βlactamases (blaVEB, blaPER, blaTEM,, blaSHV, blaCTX-M1, blaCTX-M2 and blaCTX-M3) of Pseudomonas species isolated from fish meat by direct PCR. Bacterial identification was based mainly on conventional biochemical techniques using the Vitek 2, automated system. Phenotypic sensitivity of antibiotics was established by the agar disc diffusion technique through 16 various antimicrobial drugs. Quantification of their in vitro production of numerous virulence genes outside the cell that are QS dependent namely, pyocyanin, elastase, alkaline protease, biofilm and cytotoxicity of Vero cell was as well executed. Fifteen genes demonstrated an enormous variety in their association. The total number of Pseudomonas species isolates were 30/100 to be identified by the API 20NE system as P. aeruginosa 12/30 (40%), P. fluorescens 8/30 (27%), P. putida 6/30 (20%) and P. alkylphenolia 4/30 (13%). The outcomes of this study have great significance for the strategic designation of QS quenching.
Keywords
Nile tilapia; pseudomonas; antibiotic resistance; biofilm formation; virulence genes
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.